Original Articles
Vol. 32 No. 1 (2017)
https://doi.org/10.4081/mm.2017.6510

Culturomics: bacterial species isolated in 3 healthy donors for faecal microbiota transplantation in Clostridium difficile infection

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Received: 22 December 2016
Published: 28 March 2017
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Culturomics, Faecal microbiota transplantation, Clostridium difficile

Authors

Luca Masucci
luca.masucci@policlinicogemelli.it
http://orcid.org/0000-0002-8358-6726 (unauthenticated)
Institute of Microbiology, Catholic University of the Sacred Heart, A. Gemelli Foundation, School of Medicine, Rome, Italy.
Gianluca Quaranta
Institute of Microbiology, Catholic University of the Sacred Heart, A. Gemelli Foundation, School of Medicine, Rome, Italy.
Domenico Nagel
Institute of Microbiology, Catholic University of the Sacred Heart, A. Gemelli Foundation, School of Medicine, Rome, Italy.
Sandra Primus
College for Healthcare Professions, Catholic University of the Sacred Heart, Claudiana, Bolzano, Italy.
Lucio Romano
Institute of Microbiology, Catholic University of the Sacred Heart, A. Gemelli Foundation, School of Medicine, Rome, Italy.
Rosalia Graffeo
Institute of Microbiology, Catholic University of the Sacred Heart, A. Gemelli Foundation, School of Medicine, Rome, Italy.
Gianluca Ianiro
Internal Medicine, Gastroenterology and Liver Unit, Catholic University of the Sacred Heart, School of Medicine, A. Gemelli Foundation, Rome, Italy.
Antonio Gasbarrini
Internal Medicine, Gastroenterology and Liver Unit, Catholic University of the Sacred Heart, School of Medicine, A. Gemelli Foundation, Rome, Italy.
Giovanni Cammarota
Internal Medicine, Gastroenterology and Liver Unit, Catholic University of the Sacred Heart, School of Medicine, A. Gemelli Foundation, Rome, Italy.
Maurizio Sanguinetti
Institute of Microbiology, Catholic University of the Sacred Heart, A. Gemelli Foundation, School of Medicine, Rome, Italy.
Background. Clostridium difficile infections are the main cause of nosocomial acquired diarrhea, because of prolonged antibiotic regimens. In the last years, mortality has increased due to recurrent infections caused by metronidazole and vancomicin resistant hypervirulent C. difficile strain 027. Faecal Microbiota Transplantation (FMT) is an infusion of faecal material obtained from healthy donors. This procedure reduces mortality in recurrent C. difficile infections (CDI). In this study we identified bacterial species obtained from donors’ stool samples using culturomics.
Materials and methods. Three donors were selected for FMT in three recipients affected by CDI. Faces obtained for FMT underwent culturomics, applying 17 different culture methods.
Results and conclusions. Several different bacteria were isolated from donors. In two donors 4 potentially pathogenic bacteria were isolated; this suggests the use of culturomics for donors’ screening or for selection of bacteria to infuse.

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Culturomics: bacterial species isolated in 3 healthy donors for faecal microbiota transplantation in Clostridium difficile infection. (2017). Microbiologia Medica, 32(1). https://doi.org/10.4081/mm.2017.6510

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