Scientific Articles
Vol. 20 No. 2 (2013)
https://doi.org/10.4081/microscopie.2013.4989

Balbiani granules as a model for studying RNA synthesis and processing

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Received: 16 January 2015
Published: 30 September 2013
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cell nucleus, RNA transcription, Balbiani granules.

Authors

A. Demicheli
Dipartimento di Biologia e Biotecnologie “Lazzaro Spallanzani”, Laboratorio di Biologia Cellulare e Neurobiologia, Università di Pavia, Pavia, Italy.
V. Galimberti
Dipartimento di Biologia e Biotecnologie “Lazzaro Spallanzani”, Laboratorio di Biologia Cellulare e Neurobiologia, Università di Pavia, Pavia, Italy.
M. Biggiogera
marcobig@unipv.it
Dipartimento di Biologia e Biotecnologie “Lazzaro Spallanzani”, Laboratorio di Biologia Cellulare e Neurobiologia, Università di Pavia, Pavia, Italy.
We investigated, by ultrastructural immunocytochemistry, the localization and molecular composition of Balbiani granules from Chironomus thummi polytene chromosomes. The salivary gland cells produce huge amounts of RNA coding for a single protein, hence the mRNAs have all the same length and the resulting granules share identical characteristics. Our results show the localization of cleavage, polyadenylation and splicing factors on the nascent perichromatin fibrils and, more rarely, on the already structured Balbiani granules.

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Balbiani granules as a model for studying RNA synthesis and processing. (2013). Microscopie, 20(2), 44-48. https://doi.org/10.4081/microscopie.2013.4989

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