Evaluation of two commercially available methods used for the rapid detection of ESBL-producing strains

  • Concetta De Luca
  • Erminia AM Casari
  • Elisabetta Nucleo
  • Antonella Ferrario
  • Roberta Migliavacca | r.miglia@unipv.it
  • Laura Pagani


Detection of ESBL production by Enterobacteriaceae remains a challenge for microbiologists. Although recent changes in the breakpoints of third-generation cephalosporins decreased the likelihood of reporting ESBL producers as susceptible to these compounds, ESBL detection is of interest for prevention of dissemination of ESBL-producers by cross-transmission and for epidemiological purpose. The aim of this study was to evaluate the sensitivity and the specificity of two commercial methods suitable for rapid ESBL-detection in Gram negative bacilli: the ChromID ESBL medium (bioMérieux) and the Cica-ß-Test (Mast Group, Merseyde, UK). 121 Enterobacteriaceae collected between February 2008 and April 2009 at the Laboratory Analysis IRCCS Humanitas were tested for ESBL-production by PhoenixTM, E-test (ESBL reference test), ChromID ESBL medium and Cica-β-Test. ChromID showed 100% of sensitivity and specificity for the screening of ESBL in E. coli; lower values of specificity were found in the case of P. mirabilis (81%) and Klebsiella spp. (92%). The Cica-β-Test always showed high specificity levels, but the poor sensitivity found for both E. coli (90%), P. mirabilis (73%) and Klebsiella spp. (85%), discourages its use for screening of ESBL in Gram negative bacilli from blood-cultures. Rapid identification of ESBL producers is of interest to implement hygiene precautions. In that case, using a very sensitive primary test is of major interest.



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Original Articles
ESBL, Detection, Methods
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How to Cite
De Luca, C., Casari, E. A., Nucleo, E., Ferrario, A., Migliavacca, R., & Pagani, L. (2011). Evaluation of two commercially available methods used for the rapid detection of ESBL-producing strains. Microbiologia Medica, 26(1). https://doi.org/10.4081/mm.2011.2387

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