Original Papers - Andrology
Vol. 95 No. 4 (2023)
https://doi.org/10.4081/aiua.2023.11906

Seminal calbindin 2 level in azoospermia and oligoasthenoteratozoospermia and its correlation with seminal and hormonal parameters

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Received: 2 October 2023
Accepted: 9 October 2023
Published: 15 November 2023
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Non obstructive azoospermia, Oligoasthenoteratozoospermia, normozoospermia, Seminal CALB 2 (Calretinin & 29 kDa Calbindin), Reproductive hormones

Authors

Sameh Fayek GamalEl Din
samehfayek@kasralainy.edu.eg
https://orcid.org/0000-0003-1918-5132
Department of Andrology, Sexology and STDs, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.
Noha Abdelhafeez Abdelkader
Department of Clinical and Chemical Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
Mohamed Yousry El-Amir
Department of Andrology, Sexology and STDs, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.
Asmaa Anter Sayed Ahmed
Egypt Ministry of Health & Population, Cairo, Egypt.
Hesham Fouad Abdel-latif
Department of Andrology, Sexology and STDs, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.
Mohamed Farag Azmy
Department of Andrology, Sexology and STDs, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.

Objectives: We aimed to assess seminal calbindin 2 (CALB 2) expression in men with different semen parameters as well as its correlation with reproductive hormones in azoospermic patients and different semen parameters in oligoasthenoteratozoospermic patients. CALB 2 is also known as calretinin and 29 kDa calbindin. Materials and methods: This prospective study was performed on 96 cases from the andrology outpatient clinic divided into 3 groups as follows: group 1 including 32 non obstructive azoospermic (NOA) patients, group 2 including 32 patients with oligoasthenoteratozoospermia (OAT), and Group 3 including normozoospermic individuals as controls. Semen analysis and estimation of seminal CALB 2 concentrations by enzyme linked immunosorbent assay (ELISA) technique were performed for all participants. Reproductive hormones were measured in nonobstructive NOA patients. Results: The mean seminal CALB 2 level was higher in OAT patients compared to NOA patients and controls (7.8 ± 1.30 ng/ml, 7.3 ± 0.80 and 7.4 ± 1.0, respectively). Furthermore, the study had shown strong positive correlations between CALB 2 and sperm normal forms in controls and OAT patients. In contrast, there was no significant correlation between seminal CALB 2 and any of the reproductive hormones measured in NOA patients. Conclusions: Seminal CALB 2 may play a role in increasing the abnormal forms in OAT patients.

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Seminal calbindin 2 level in azoospermia and oligoasthenoteratozoospermia and its correlation with seminal and hormonal parameters. (2023). Archivio Italiano Di Urologia E Andrologia, 95(4). https://doi.org/10.4081/aiua.2023.11906
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