Original Articles
Vol. 30 No. 1 (2015)
https://doi.org/10.4081/mm.2015.5015

Phenotypic classification of multidrug-resistant Klebsiella through MALDI-TOF mass spectrometry

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Received: 21 January 2015
Published: 18 June 2015
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Klebsiella pneumoniae, KPC, MALDI-TOF MS

Authors

Anna Di Taranto
aditaranto@ospedaliriunitifoggia.it
Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
Rosella De Nittis
Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
Marco Fagioni
Bruker Daltonics s.r.l., Macerata, Italy.
Tiziana Rollo
Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
Paola Cella
Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
Raffaele Antonetti
Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
Background. Resistance to carbapenems in Klebsiella pneumoniae infections could compromise therapy efficiency in hospitals. In Italy, the percentage of K. pmeumoniae resistant to carbapenems blood-isolated is 35%.
Materials and Methods. Seventy-three multidrug-resistant K. pneumoniae clinical isolates coming from Foggia (Italy) have been phenotypically classified through matrix assisted laser desorption/ionization-time of flight mass spectrometry between February and May 2014 in order to locate an eventual infectious outbreak.
Conclusions. Classification of microbial isolates is essential to this purpose and starts from phenotypic methodologies to the most complex genotypic methods. Among these, mass spectrometry utilization appears to be a simple, useful and quick instrument in a clinical microbiology laboratory.

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Phenotypic classification of multidrug-resistant Klebsiella through MALDI-TOF mass spectrometry. (2015). Microbiologia Medica, 30(1). https://doi.org/10.4081/mm.2015.5015

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