Original Articles
Vol. 31 No. 1 (2016)
https://doi.org/10.4081/mm.2016.4825

Impact of two different commercial DNA extraction methods on BK virus viral load

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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 11 November 2014
Published: 31 March 2016
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DNA extraction, BKV viral load, PCR real time, transplant recipients

Authors

Massimiliano Bergallo
massimiliano.bergallo@unito.it
Department of Public Health and Paediatric Sciences, University of Turin Medical School, Turin; Laboratory of Citoimmunodiagnostics, University Hospital of City Science and Health, Regina Margherita Children's Hospital, Turin, Italy.
Ilaria Galliano
Department of Public Health and Paediatric Sciences, University of Turin Medical School, Turin; Laboratory of Citoimmunodiagnostics, University Hospital of City Science and Health, Regina Margherita Children's Hospital, Turin, Italy.
Elisa Loiacono
Nephrology, Dialysis and Transplantation, University Hospital City of Science and Health, Regina Margherita Children's Hospital, Turin, Italy.
Francesca Ferro
Laboratory of Citoimmunodiagnostics, University Hospital of City Science and Health, Regina Margherita Children's Hospital, Turin, Italy.
Paola Montanari
Department of Public Health and Paediatric Sciences, University of Turin Medical School, Turin; Laboratory of Citoimmunodiagnostics, University Hospital of City Science and Health, Regina Margherita Children's Hospital, Turin, Italy.
Paolo Ravanini
Laboratory of Molecular Virology, Azienda Ospedaliero-Universitaria Maggiore della Carità, Novara, Italy.
Background and aim: BK virus, a member of human polyomavirus family, is a worldwide distributed virus characterized by a seroprevalence rate of 70-90% in adult population. Monitoring of viral replication is made by evaluation of BK DNA by quantitative polymerase chain reaction. Many different methods can be applied for extraction of nucleic acid from several specimens. The aim of this study was to assess the impact of two different DNA extraction procedure on BK viral load.
Materials and methods: DNA extraction procedure including the Nuclisens easyMAG platform (bioMerieux, Marcy l’Etoile, France) and manual QIAGEN extraction (QIAGEN Hilden, Germany). BK DNA quantification was performed by Real Time TaqMan PCR using a commercial kit.
Result and discussion: The samples capacity, cost and time spent were compared for both systems. In conclusion our results demonstrate that automated nucleic acid extraction method using Nuclisense easyMAG was superior to manual protocol (QIAGEN Blood Mini kit), for the extraction of BK virus from serum and urine specimens.

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Impact of two different commercial DNA extraction methods on BK virus viral load. (2016). Microbiologia Medica, 31(1). https://doi.org/10.4081/mm.2016.4825

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