https://doi.org/10.4081/mm.2025.13617
Molecular epidemiology of Pseudomonas aeruginosa in intensive care units
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Published: 30 July 2025
Background: Pseudomonas aeruginosa is a major nosocomial pathogen that is hard to treat because of its multiple antibiotic resistance. In this study, we aimed to investigate the molecular epidemiology and antibiotic susceptibility of clinical P. aeruginosa isolates from Intensive Care Unit (ICU) patients in order to contribute to developing rational antibiotic usage policies and monitoring infection control precautions.
Materials and Methods: seventy-five P. aeruginosa isolates from clinical specimens of patients in ICUs of Erciyes University Medical Faculty Hospital were studied. Bacterial identification was made using conventional methods and/or an automated Phoenix ID system (BD Diagnostics, Franklin Lakes, USA). The Minimal Inhibitory Concentration (MIC) for antibiotics was determined by broth microdilution. Repetitive sequence-based Polymerase Chain Reaction (rep-PCR) was used to assess the clonal relationship of the strains.
Results: the resistance rates of the strains for amikacin, gentamicin, ciprofloxacin, levofloxacin, ceftazidime, cefepime, imipenem, meropenem, colistin were 2.7%,18.7%, 24%, 22.7%, 16%, 5.3%, 64%, 60%, and 1.3% respectively. According to rep-PCR results, 21 clones (A-U) were identified. It was found that 33.3% of the strains were in clone B, which was the dominant type. It was found that antibiotic susceptibility patterns of some clonally related isolates were similar.
Conclusions: it is important to monitor resistance rates of infectious agents for guiding clinicians in empirical therapy and making rational antibiotic usage policies, it is also important to investigate clonal relationships for tracing the spread of infectious agents, especially multidrug-resistant pathogens and infection control precautions.
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