Detection of free-living amoeba in a tertiary care hospital

Submitted: 15 January 2024
Accepted: 22 March 2024
Published: 21 May 2024
Abstract Views: 542
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This study aims to investigate the presence of Free-Living Amoebae (FLA), particularly Acanthamoeba spp., in diverse water systems and body fluids within a hospital. Water and body fluid samples were collected from various locations. Cultivation of FLA was performed using Non-Nutrient Agar (NNA) culture with Escherichia coli, and microscopic observations after staining. Microscopic observations revealed the presence of Acanthamoeba spp. in pre-dialysis tank water and dental flush water samples. The identification of Acanthamoeba in specific water sources underscores the importance of continuous monitoring and preventive strategies to mitigate potential health risks.

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Aparicio DV, Bejarano JIC, de Los Santos AM, et al. Case Report: Granulomatous Amebic Encephalitis due to Acanthamoeba spp. in an Immunocompetent Pediatric Patient. The American Journal of Tropical Medicine and Hygiene 2021;105:167-70. DOI: https://doi.org/10.4269/ajtmh.21-0129
Arberas-Jiménez I, Sifaoui I, Reyes-Batlle M, et al. Ultraviolet – Chlorine combined treatment efficiency to eliminate Naegleria fowleri in artificial surf lagoons. Heliyon 2022;8:e11625. DOI: https://doi.org/10.1016/j.heliyon.2022.e11625
Berger JR. Amebic infections of the central nervous system. Journal of NeuroVirology 2022;28:467-72. DOI: https://doi.org/10.1007/s13365-022-01096-x
Borella Da Silva TC, Dos Santos DL, Rott MB. First report of free-living amoebae in sewage treatment plants in Porto Alegre, southern Brazil. Journal of Water and Health 2023;21:1611-24. DOI: https://doi.org/10.2166/wh.2023.261
Borin S, Feldman I, Ken-Dror S, Briscoe D. Rapid diagnosis of acanthamoeba keratitis using non-nutrient agar with a lawn of E. coli. Journal of Ophthalmic Inflammation and Infection 2013;3:40. DOI: https://doi.org/10.1186/1869-5760-3-40
Chaúque BJM, Dos Santos DL, Anvari D, Rott MB. Prevalence of free-living amoebae in swimming pools and recreational waters, a systematic review and meta-analysis. Parasitology Research 2022;121:3033-50. DOI: https://doi.org/10.1007/s00436-022-07631-3
De Jonckheere JF. Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri. Infection, Genetics and Evolution 2011;11:1520-8. DOI: https://doi.org/10.1016/j.meegid.2011.07.023
Delafont V, Rodier M-H, Maisonneuve E, Cateau E. Vermamoeba vermiformis: a Free-Living Amoeba of interest. Microbial Ecology 2018;76:991-1001. DOI: https://doi.org/10.1007/s00248-018-1199-8
Jahangeer M, Mahmood Z, Munir N, et al. Naegleria fowleri: Sources of infection, pathophysiology, diagnosis, and management; a review. Clinical and Experimental Pharmacology and Physiology 2020;47:199-212. DOI: https://doi.org/10.1111/1440-1681.13192
Kalra SK, Sharma P, Shyam K, et al. Acanthamoeba and its pathogenic role in granulomatous amebic encephalitis. Experimental Parasitology 2020;208:107788. DOI: https://doi.org/10.1016/j.exppara.2019.107788
Kaushal V, Chhina DK, Ram S, et al. Primary amoebic meningoencephalitis due to Naegleria fowleri. The Journal of the Association of Physicians of India 2008;56:459-62.
Khan NA. Acanthamoeba invasion of the central nervous system. International Journal for Parasitology 2007;37:131-8. DOI: https://doi.org/10.1016/j.ijpara.2006.11.010
Khatoonaki H, Solhjoo K, Rezanezhad H, et al. Isolation and identification of potentially pathogenic Free-Living Amoeba in dental-unit water samples. Journal of Water and Health 2022;20:1126-36. DOI: https://doi.org/10.2166/wh.2022.097
Khurana S, Biswal M, Kaur H, et al. Free-living amoebae in water sources of critical units in a tertiary care hospital in India. Indian Journal of Medical Microbiology 2015;33:343-8. DOI: https://doi.org/10.4103/0255-0857.158543
Khurana S, Sharma C, Radotra BD, et al. Molecular diagnosis of encephalitis/meningoencephalitis caused by Free-Living Amoebae from a tertiary center in India. Pathogens 2022;11:1509. DOI: https://doi.org/10.3390/pathogens11121509
Kot K, Łanocha-Arendarczyk N, Kosik-Bogacka D. Immunopathogenicity of Acanthamoeba spp. in the brain and lungs. International Journal of Molecular Sciences 2021;22:1261. DOI: https://doi.org/10.3390/ijms22031261
Krishnamoorthi S, Sharma C, Mewara A, Khurana S. Environmental water surveillance for free-living amoeba in North India. Indian Journal of Medical Microbiology 2022;40:389-93. DOI: https://doi.org/10.1016/j.ijmmb.2022.05.002
Lasjerdi Z, Niyyati M, Haghighi A, et al. Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran. Parasitol Res 2011;109:575-80. DOI: https://doi.org/10.1007/s00436-011-2288-5
Milanes JE, Suryadi J, Monaghan NP, et al. Characterization of glucokinases from pathogenic Free-Living Amoebae. Antimicrobial Agents and Chemotherapy 2022;66:e02373-21. DOI: https://doi.org/10.1128/aac.02373-21
Miller HC, Wylie JT, Kaksonen AH, et al. Competition between Naegleria fowleri and Free-Living Amoeba colonizing laboratory scale and operational drinking water distribution systems. Environmental Science & Technology 2018;52:2549-57. DOI: https://doi.org/10.1021/acs.est.7b05717
Mrva M, Garajová M. The efficiency of cultivation media in recovering naked lobose amoebae from freshwater environments. Zoological Studies 2018;57:e9.
Panda A, Khalil S, Mirdha BR, et al. Prevalence of Naegleria fowleri in Environmental Samples from Northern Part of India. PLOS ONE 2015;10:e0137736. DOI: https://doi.org/10.1371/journal.pone.0137736
Panda A, Mirdha BR, Rastogi N, Kasuhik S. Understanding the true burden of “Naegleria fowleri” (Vahlkampfiidae) in patients from Northern states of India: source tracking and significance. European Journal of Protistology 2020;76:125726. DOI: https://doi.org/10.1016/j.ejop.2020.125726
Raju R, Khurana S, Mahadevan A, John DV. Central nervous system infections caused by pathogenic free-living amoebae: an Indian perspective. Tropical Biomedicine 2022;39:265-80. DOI: https://doi.org/10.47665/tb.39.2.017
Reyes-Batlle M, Díaz FJ, Sifaoui I, et al. Free-Living Amoebae isolation in irrigation waters and soils of an insular arid agroecosystem. Science of The Total Environment 2021;753:141833. DOI: https://doi.org/10.1016/j.scitotenv.2020.141833
Sadaka HA, Emam EE. Is the intestinal tract a portal of entry for Acanthamoeba infection? Journal of the Egyptian Society of Parasitology 2001;31:781-90.
Samba-Louaka A, Delafont V, Rodier M-H, et al. Free-living amoebae and squatters in the wild: Ecological and molecular features. FEMS Microbiology Reviews 2019;43:415-34. DOI: https://doi.org/10.1093/femsre/fuz011
Shi Y, Queller DC, Tian Y, et al. The ecology and evolution of amoeba-bacterium interactions. Applied and Environmental Microbiology 2021;87:e01866-20. DOI: https://doi.org/10.1128/AEM.01866-20
Siddiqui R, Makhlouf Z, Khan NA. The increasing importance of Vermamoeba vermiformis. Journal of Eukaryotic Microbiology 2021;68:e12857. DOI: https://doi.org/10.1111/jeu.12857
Soares SS, Souza TK, Berté FK, et al. Occurrence of infected Free-Living Amoebae in cooling towers of Southern Brazil. Current Microbiology 2017;74:1461-8. DOI: https://doi.org/10.1007/s00284-017-1341-8
Tananuvat N, Techajongjintana N, Somboon P, Wannasan A. The first acanthamoeba keratitis case of non-contact lens wearer with hiv infection in Thailand. The Korean Journal of Parasitology 2019;57:505-11. DOI: https://doi.org/10.3347/kjp.2019.57.5.505
Trabelsi H, Dendana F, Sellami A, et al. Pathogenic free-living amoebae: Epidemiology and clinical review. PathologieBiologie 2012;60:399-405. DOI: https://doi.org/10.1016/j.patbio.2012.03.002
Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic Free-Living Amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappiniadiploidea. FEMS Immunology & Medical Microbiology 2007;50:1-26. DOI: https://doi.org/10.1111/j.1574-695X.2007.00232.x
Wopereis D, Bazzo M, De Macedo J, et al. Free-Living Amoebae and their relationship to air quality in hospital environments: characterization of Acanthamoeba spp. obtained from air-conditioning systems. Parasitology 2020;147:782-90. DOI: https://doi.org/10.1017/S0031182020000487
Yazar S, Gürbüz E, Sönmez MF, et al. Investigation of potentially pathogenic Free-Living Amoebae and their in vivo pathogenicity in water supplies of Turkey. MikrobiyolojiBulteni 2016;50:449-59. DOI: https://doi.org/10.5578/mb.27898

How to Cite

Choudhuri, N., Chaudhuri, B. N., Guchhait, P., Dawn, A. K., & Das, S. (2024). Detection of free-living amoeba in a tertiary care hospital. Microbiologia Medica, 39(1). https://doi.org/10.4081/mm.2024.12287