Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria
https://doi.org/10.4081/mm.2023.11445
Accepted: 11 September 2023
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Background and Aims: Staphylococcus aureus is one of the most important pathogens of public health concern and a leading cause of nosocomial infections. In this study, we evaluated the effect of routinely used disinfectants in hospitals for surface decontamination on biofilm-forming S. aureus.
Materials and Methods: forty-eight S. aureus isolates were phenotypically evaluated for biofilm formation using the Tissue Culture Plate (TCP) technique. Effect of disinfectants (Dettol®, Izal®, Jik® and Savlon®) on biofilm was tested and time-kill kinetics evaluated. PCR was used to confirm the identity of S. aureus using species-specific primers.
Results: biofilm formation assay revealed that 15 (31.2%) of the isolates formed biofilm with 7 (14.5%) and 8 (16.6%) considered as strong and moderate biofilm formers, respectively. Biofilm formation was time-dependent (p<0.0001). Jik® was significantly effective (p<0.0001) as it disrupted biofilm formed in all 15 (100%) isolates, followed by Izal® 13 (86.6%), Savlon® 11 (73.3%) and Dettol® 9 (60%). Time-kill kinetics of the four disinfectants revealed Dettol®, Jik® and Savlon® achieved total (100%), (7 log10) lethality against isolates within 1 h contact time while Izal® attained complete lethality at 6 h contact time.
Conclusions: of the four disinfectants evaluated Jik®, a chlorine- based formulation, was more effective in destroying biofilmforming S. aureus. The need to use effective disinfectants in sanitization is imperative to facilitate the control and prevention of hospital and community-acquired infections.
Abdulrahim U, Kachallah M, Rabiu M, et al. Microbiology Molecular Detection of Biofilm-Producing Staphylococcus aureus Isolates from National Orthopaedic Hospital Dala, Kano State, Nigeria. J Med Microbiol 2019;9:116-26. DOI: https://doi.org/10.4236/ojmm.2019.93012
Aiyegoro OA, Afolayan AJ, Okoh AI. In vitro antibacterial time kill studies of leaves extracts of Helichrysum longifolium. J Med Plant Res 2009;3:462-7.
Brooks G, Butel S, Morse S. Medical Microbiology. 23rd ed. McGraw Hill Professional, Singapore. 2004.
Buckingham-Meyer K, Goeres DM, Hamilton MA. Comparative evaluation of biofilm disinfectant efficacy tests. J Microbiol Methods 2007;70:236-44. DOI: https://doi.org/10.1016/j.mimet.2007.04.010
CDC and ICAN. Best Practices for Environmental Cleaning in Healthcare Facilities in Resource-Limited Settings. Atlanta, GA: US Department of Health and Human Services, CDC; Cape Town, South Africa: Infection Control Africa Network. 2019. Available from: https://www.cdc.gov/hai/prevent/resource-limited/index.html
Cheesbrough M. Staphylococcus aureus. In: District laboratory practice in tropical countries. Part 2. 2nd Edition. Cambridge University Press, London, UK. 2006. DOI: https://doi.org/10.1017/CBO9780511543470
Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime–producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1987;37:318-26. DOI: https://doi.org/10.1128/iai.37.1.318-326.1982
Costa S, Elisabete J, Cláudia P, et al. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus. J Microbiol 2013;7:59-71. DOI: https://doi.org/10.3390/antibiotics2010083
Dantes R, Mu Y, Belflower R, et al. Emerging infections program–active bacterial Core surveillance MRSA surveillance investigators. National burden of invasive methicillin-resistant Staphylococcus aureus infections, United States. JAMA Intern Med 2013;173:1970-8. DOI: https://doi.org/10.1001/jamainternmed.2013.10423
de Morais S, Kak G, Menousek P, Kielian T. Immunopathogenesis of Craniotomy Infection and Niche-Specific Immune Responses to Biofilm. Front Immunol 2021;12:625467. DOI: https://doi.org/10.3389/fimmu.2021.625467
EL Mahmood AM, Doughari JH. Effect of Dettol on viability of some microorganisms associated with nosocomial infections. J Afric Biotechnol 2008;7:1554-62.
Eyo AO, Ibeneme EO, Ogba OM, Asuquo AE. Antibacterial Efficacy of the In-Use Dilutions of Common Disinfectants against Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital in Calabar, Nigeria. J Pharm Biol Sci 2018;13:88-91.
Hugo WA, Bloomfield SF. Studies on the mode of action of phenolic antibacterial agent fenticlor against Staphylococcus aureus and Escherichia coli 1. Adsorption of fenticlor by the bacterial cell and its antibacterial activity. J Appl Bacteriol 1971;34:557-67. DOI: https://doi.org/10.1111/j.1365-2672.1971.tb02318.x
Iniguez-Moreno M, Gutiérrez-Lomelí M, Guerrero-Medina PJ, Avila-Novoa MG. Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. Brazilian J Microbiol 2018;49:310-9. DOI: https://doi.org/10.1016/j.bjm.2017.08.002
Inyang CU, Fatunla OK, Akpan AS. Effect of Exposure Time on the Antibacterial Activity of Disinfectants Used in Uyo Abattoirs. World J Appl Sci Technol 2018;10:163-8.
Kara I, Hassaine H, Kara A, et al. Effects of certain disinfectants and antibiotics on biofilm formation by Staphylococcus aureus isolated from medical devices at the University Hospital Center of Sidi Bel Abbes, Algeria. Afr J Clin Exper Microbiol 2020;21:304-10. DOI: https://doi.org/10.4314/ajcem.v21i4.6
Lineback CB, Carine A, Nkemngong L, et al. Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds. Antimicrob Resis Infect Control 2018;7:154. DOI: https://doi.org/10.1186/s13756-018-0447-5
Lotfipour F, Nahaei MR, Milani M, et al. Antibacterial Activity of Germicide-PÆ: A Persulfate Based Detergent/Disinfectant on Some Hospital Isolates. Iranian J Pharmaceut Sci 2006;2:225-30.
Mathur T, Singhal S, Khan S, et al. Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol 2006;24:25-9. DOI: https://doi.org/10.1016/S0255-0857(21)02466-X
Murray PR, Rosenthal K, Kobayashi S, Pfaller M. Medical Microbiology, 4th edition. Elsevier, Amsterdam, The Netherlands. 2002. 872 pp.
Oleghe P, Agholor K, Lucy O, et al. Comparative antimicrobial study of a locally produced disinfectant and some commercially available disinfectants against some clinical isolates. J World Pharmaceut Life Sci 2020;6:01-06.
Orjih CI, Ajayi A, Alao FO, et al. Characterization of biofilm in clinical urinary isolates of Staphylococcus aureus from five hospitals in Lagos State, Nigeria. Afr J Exper Microbiol 2021;22:164-9. DOI: https://doi.org/10.4314/ajcem.v22i2.8
Russell AD. Activity of biocides against mycobacteria. J Appl Bacteriol Symp 1996;81:87-101. DOI: https://doi.org/10.1111/j.1365-2672.1996.tb04837.x
Sunagar SN, Deore PV, Deshpande A, et al. Differentiation of Staphylococcus aureus and Staphylococcus epidermidis by PCR for the fibrinogen binding protein gene. J Dairy Sci 2013;96:2857-65. DOI: https://doi.org/10.3168/jds.2012-5862
Tahaei SSA, Stájer A, Barrak I, et al. Correlation Between Biofilm-Formation and the Antibiotic Resistant Phenotype in Staphylococcus aureus Isolates: A Laboratory-Based Study in Hungary and a Review of the Literature. Infect Drug Resist 2021;14:1155-68. DOI: https://doi.org/10.2147/IDR.S303992
Tiwari S, Rajak S, Mondal D, Debasis B. Sodium hypochlorite is more effective than 70% ethanol against biofilms of clinical isolates of Staphylococcus aureus. American J Infect Contrl 2018;46:37-42. DOI: https://doi.org/10.1016/j.ajic.2017.12.015
Torlaka E, Korkut E, Uncua AT, Sener Y. Biofilm formation by Staphylococcus aureus isolates from a dental clinic in Konya, Turkey. J Infect Public Health 2017;10:809-13. DOI: https://doi.org/10.1016/j.jiph.2017.01.004
Uchejeso OM. Time Kill Kinetics Study of Commonly Used Disinfectants against Biofilm forming Pseudomonas aeruginosa in Federal Medical Centre, Umuahia-Nigeria. Am J Biomed Sci Res 2020;7:3. DOI: https://doi.org/10.34297/AJBSR.2020.07.001155
White RL, Burgess DS, Manduru M, Bosso JA. Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E-test. Antimicrob Agents Chemother 1996;40:1914-8. DOI: https://doi.org/10.1128/AAC.40.8.1914
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