Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae

Submitted: 2 September 2016
Accepted: 30 January 2017
Published: 21 April 2017
Abstract Views: 2678
PDF: 919
HTML: 902
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.


For effective vector control it is essential to formulate new preparations having multiple action against the vector pest. Developing combined formulation of biopesticide and chemical pesticide is one of the novel concept to fight against the vectors with new weapons; however, compatibility of biopesticide i.e. Bacillus thuringiensis (Bt) and chemical pesticide is a real hurdle. In this investigation, local isolate Bacillus thuringiensis SV2 (BtSV2) was tested for its compatibility with various available mosquito larvicides. Temephos was most compatible with BtSV2 than with other tested pesticides. These two compatible agents were tested for larvicidal potential. Our study revealed that the synergistic effect of both agents reduces LC50 value by 30.68 and 22.36% against the Ae. aegypti and An. stephensi, respectively. The larvicidal potential increased when compared to individual pesticides. It was also observed a biochemical change in larvae after the TBT (Temephos + Bacillus thuringiensis) combination treatment; it involves decreased level of alpha esterase, acetylcholine esterase and protein while level of beta esterase and acid phosphatase was unchanged and alkaline phosphatase activity was increased. Increased potential of combined formulation may be due to altered physiological condition.



PlumX Metrics


Download data is not yet available.


Supporting Agencies


How to Cite

Narkhede, C., Patil, C., Suryawanshi, R., Koli, S., Mohite, B., & Patil, S. (2017). Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae. Journal of Entomological and Acarological Research, 49(1).