Efficacy of Eucalyptus citriodora and Syzygium aromaticum essential oil as insecticidal, antiovipositant, and fumigant against Callosobruchus maculatus F (Coleoptera: Bruchidae)

Published: 12 September 2023
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Callosobruchus maculatus is the most pernicious pest of stored grain worldwide. Even though synthetic insecticides are commonly used to eliminate this insect pest, the negative effect of this pest management method on humans and the environment raises concern among people around the world. This study was done to identify the active ingredient of essential oils in Eucalyptus citriodora and Syzygium aromaticum and to evaluate the effectiveness of those essential oils in controlling C. maculatus. The results of gas chromatography/mass spectrometry analysis indicated that the essential oil extracted from the leaves of S. aromaticum are rich in Eugenol and β caryophyllene as much as 81% and 14.65% consecutively, while E. citriodora oil contains 86% of Citronella. According to the bioassay results, increasing the essential oil concentration from 1% to 3% resulted in a significant increase in insect mortality rate, oviposition deterrence, and fumigant toxicity. Additionally, S. aromaticum has significantly shown a higher insecticidal performance compared to E. citriodora. However, there are no synergistic effects observed on the use of essential oil of both plant species on C. maculatus. These results suggest that S. aromaticum and E. citriodora essential oils could be potential candidates as a natural insecticide in managing C. maculatus in stored products.



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ALMA H.A., ERTAS M., NITZ S., KOLLMANNSBERGER H., 2007 – Chemical composition and content of essential oil from the bud of cultivated Turkish Clove (Syzygium aromaticum L.). – BioResources, 2(2): 265-269. DOI: https://doi.org/10.15376/biores.2.2.265-269
ASTUTHI M.M.M., SUMIARTHA K., SUSILA I.W., WIRYA G.N.A.S., SUDIARTA I.P., 2012 – Efficacy of clove oil (Syzigium aromaticum), ginger oil (Zingiber officinale), and nutmeg oil (Myristica fragrans) to hairy caterpillar Fam: Lymantriidae). – J. Agric. Sci. Biotechnol., 1(1): 12-23.
AHUCHAOGU C.E., OJIAKO F.O., 2020 – Attraction of Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae: Bruchinae) to pods of cowpea plants at different developmental stages. – J. Trop. Agric. Food Environ. Exten., 19:32-39. DOI: https://doi.org/10.4314/as.v19i3.6
BAKER B.P., GRANT J.A., KUENEN R.M., 2018 – Active ingredient eligible for minimum risk pesticide use. – Available from http://hdl.handle.net/1813/56120
BAO L.T., QI Z.L., ZHI L.L., PENG L., JIE W.W., 2015 – Insecticidal potential of clove essential oil and its constituents on Cacopsylla chinensis (Hemiptera: Psyllidae) in laboratory and field. – J. Econ. Entomol., 108(3): 57-61. DOI: https://doi.org/10.1093/jee/tov075
BARCELOUX D.G., 2008 – Medical toxicology of natural substances: Foods, fungi, medicinal herbs, plants, and venomous animals – Wiley: Hoboken. New Jersey. DOI: https://doi.org/10.1002/9780470330319
BENCHAA S., HAZZIT M., ABDELKRIMA H., 2018 – Allelopathic effect of Eucalyptus citriodora essential oil and its potential use as bioherbicide. – Chem. Biodiver. J., 15:1-9. DOI: https://doi.org/10.1002/cbdv.201800202
BECK C.W., BLUMER L.S., 2011 – A handbook on bean beetles Callosobruchus maculatus. – National Science Foundation, 156 pp.
DAIZY R.B., HARMINDER P.S., RAVINDER K.K., SHALINDER K., 2008 – Eucalyptus essential oil as a natural pesticide. – J. For. Ecol. Manag., 256: 2166-2174. DOI: https://doi.org/10.1016/j.foreco.2008.08.008
DEVI M.D., DEVI N.V., 2014 – Biology and morphometric measurement of cowpea weevil, Callosobruchus maculatus fabr. (Coleoptera: Chrysomelidae) in green gram. – J. Entomol. Zool Stud., 2 (3): 74-76.
EMEASOR K.C., CHUKWU L.A., 2019 – Evaluation of toxicity of some plant materials against the bruchid (Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) on Mung Bean (Vigna radiata (L.) Wilczek) seeds in storage. – Acta Sci. Agric. J., 3: 23-26. DOI: https://doi.org/10.31080/ASAG.2019.03.0634
GOBAIA M., OKTAVIANUS., ROCHMAN N., 2015 – Insecticidal effect of Otikai leaf extract (Alphitonia sp.) and Pinang Fruit Extract (Areca catechu L.) on the mortality of Callosobruchus chinensis L. – J. Agronida, 1(2): 71-82.
GONZÁLEZ H.J.N., HERRERA C.G.A., VELÁZQUEZ M.M., ANDREWS H.E., 2021 – Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications and essential bioactivities for human health. – Molecules, 26(21): 6387. DOI: https://doi.org/10.3390/molecules26216387
HARRIS R., 2002 – Synergism in the essential oil world. – Int. J. Aromather., 12(4):179-186. DOI: https://doi.org/10.1016/S0962-4562(02)00083-8
HARTSEL J.A., EADES J., HICKORY B., MAKRIYANNIS A., 2016 – Cannabis sativa and Hemp in Nutraceuticals efficacy, safety and toxicity. – Gupta, R.C., Lall R., Srivastava A., (Eds). Nutraceuticals: efficacy, safety and toxicity. Academic Press. Available from: https://doi.org/10.1016/B978-0-12-802147-7.00053-X DOI: https://doi.org/10.1016/B978-0-12-802147-7.00053-X
HASYIM A., SETIAWATI W., JAYANTI H., KRESTINI E.H., 2014 – Repellency of essential oils against Ephestia cautella (Walker) (Lepidoptera: Pyrallidae) under laboratory condition. – J. Hortic., 24(4):336-345. DOI: https://doi.org/10.21082/jhort.v24n4.2014.p336-345
HASEGAWA T., TAKANO F., TAKATA T., NIIYAMA M., OHTA T., 2008 – Bioactive monoterpene glycosides conjugated with gallic acid from the leaves of Eucalyptus globulus. – Phytochemistry, 69(3):747-53. DOI: https://doi.org/10.1016/j.phytochem.2007.08.030
KACHKOUL R., TOUIMI G.B., BENNANI B., HABBANI E.R., MOUHRI G.E., MOHIM M., HOUSSAINI T.S., CHEBAIBI M., KOULOU A., LAHRICHI A., 2021 – The synergistic effect of three essential oils against bacteria responsible for the development of lithiasis infection: An optimization by the mixture design. – Evid. Based Complement. Alternat. Med., ID: 1305264. DOI: https://doi.org/10.1155/2021/1305264
KALIRAMESH S., CHELLADURAI V., JAYAS D., ALAGUSUNDARAM K., WHITE N., FIELDS P.G., 2013 – Detection of infestation by Callosobruchus maculatus in mung bean using near-infrared hyperspectral imaging. – J. Stored Prod. Res., 52:107-111. DOI: https://doi.org/10.1016/j.jspr.2012.12.005
KEBE K., ALVAREZ N., ESPINDOLA A., 2020 – Oviposition choice and larval development of the seed beetle Callosobruchus maculatus (F) (Coleoptera : Chrysomelidae: Bruchinae) on three cowpea varieties. – J. Stored Prod. Res., 86: 1-6. DOI: https://doi.org/10.1016/j.jspr.2020.101578
KHALIL A.A., RAHMAN U., KHAN M.R., SAHAR A., MEHMOOD T, KHAN M., 2017 – Essential oil eugenol: Sources, extraction techniques and nutraceutical perspectives. - RSC Advances, 7: 32669-32681. DOI: https://doi.org/10.1039/C7RA04803C
KHAN I.A., ABOURASHED E.A., 2010 – Leung’s Encyclopaedia of Common Natural Ingredients Used in Food, Drugs, and Cosmetics. – 3rd ed. Hoboken, N.J.: John Wiley and Sons.
KHAN J., KHAN I., QAHAR A., SALMAN M., ALI F., SALMAN M., KHAN K., HUSSAIN F., ABBASI A., 2017 – Efficacy of citronella and eucalyptus oils against Musca domestica, Cimex lectularius and Pediculus humanus. – Asian Pac. J. Trop. Dis., 7(11): 691-695. DOI: https://doi.org/10.12980/apjtd.7.2017D7-2
MEENA T., GUPTA D., 2013 – Plant extracts as oviposition deterrents against fruit flies, Bactrocera spp. – Pestic. Res. J., 25(1): 24-28.
MUHAMMAD A., BASHIR A.K, DUTSIN M.A., 2017 – Callosobruchus maculatus (Fab.) control by plant products in cowpea grains under storage. – J. Med Botany, 1: 51-57. DOI: https://doi.org/10.25081/jmb.2017.v1.897
MAHENDRAN K., MOHAN S., 2002 – Technology adoption, estimation of loss and farmers behavior in pulses storage. A study in Western Tamil Nadu. – Pestology, 26:35-38.
NAJLA D., ONS M., SALAHEDDINE S., WAFA T., ASMA L., IKBAL C., 2013 – Chemical composition and bio-insecticidal activity of Eugenia caryophyllata essential oil against Spodoptera littoralis. – Microbiol. Hyg. Alim, 25: 94-98.
NISAR M.S., HAQ I.U., RAMZAN H., ALJEDANI D.H., QASIM M., ISLAM W., KHAN K.A., 2021 – Screening of different legumes for the developmental preference of Callosobruchus maculatus (Bruchidae: Coleoptera). – Int J. Trop. Insect Sci., 41(3):1-8. DOI: https://doi.org/10.1007/s42690-021-00507-6
NURDJANNAH N., BERMAWIE N., 2012 – Cloves. – In: Peter KV (Eds), Handbook of Herbs and Spices. Woodhead Publishing. DOI: https://doi.org/10.1533/9780857095671.197
SARWAR M., 2012 – Assessment of resistance to the attack of bean beetle Callosobruchusm maculatus (Fabricius) in chickpea genotypes on the basis of various parameters during storage. – J. Sci. Technol., 34 (3): 287-291.
SELLES S.M.A., KOUIDRI M., BELHAMITI B.T., AMRANE A.A., 2020 – Chemical composition, in-vitro antibacterial and antioxidant activities of Syzygium aromaticum essential oil. – J. Food Meas. Charact., 14:2352-2358. DOI: https://doi.org/10.1007/s11694-020-00482-5
SHARMA D.K., KAUR J., 2017 – Efficacy of traditional products on the biochemical aspects of stored mungbean infested with Callosobruchus maculatus (Fabricius). – Appl. Nat. Sci. J., 9 (4): 2110-2113. DOI: https://doi.org/10.31018/jans.v9i4.1496
SHEVKANI K., SINGH N., CHEN Y., KAUR A., YU L., 2019 – Pulse proteins: Secondary structure, funcionality and applications. – J. Food. Sci. Technol., 56: 2787-2798. DOI: https://doi.org/10.1007/s13197-019-03723-8
THEIN N.S., ARAN N., WISUT S., 2020 – Bioactivity of some plant essential oils for seed treatment against pulse beetle, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) on mung bean. – Bulg. J. Agric. Sci., 26 (1): 141-147.
TUNI I., SAHINKAYA S., 1998 – Sensitivity of two greenhouse pests to vapours of essential oils. – Entomol. Exp. App., 86: 183-187. DOI: https://doi.org/10.1046/j.1570-7458.1998.00279.x
ZAKARAYA D., FKIH-TETOUANI S., HAJJI F., 1993 – Chemical composition-antimicrobial relationships of Eucalyptus essential oils. – Plantes Medicinales et Phytotherapie, 4: 319-331.

How to Cite

Kardinan, A., Maris, P., Darwati, I., Mustapha, Z., & Ngah, N. (2023). Efficacy of <em>Eucalyptus citriodora</em> and <em>Syzygium aromaticum</em> essential oil as insecticidal, antiovipositant, and fumigant against <em>Callosobruchus maculatus</em> F (Coleoptera: Bruchidae). Journal of Entomological and Acarological Research, 55(1). https://doi.org/10.4081/jear.2023.11670