Ultrasensitive MAPK/Erk activation in absence of protein synthesis in Xenopus oocytes

  • Rémy Beaujois | jean-francois.bodart@univ-lille1.fr EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France.
  • Franck Riquet EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France.
  • Katia Cailliau EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France.
  • Edith Browaeys-Poly EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France.
  • Christophe Russo EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France.
  • Ralf Blossey USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France.
  • Dorothée Vicogne USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France.
  • Matthieu Marin EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France.
  • Arlette Lescuyer-Rousseau EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France.
  • Jean-Pierre Vilain EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France.
  • Jean-François Bodart EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France.

Abstract

The mitogen-activated protein kinase (MAPK) cascade in Xenopus oocytes exhibits an all-or-none, ultrasensitive response, which is believed to result from a positive feed-back loop. Here we describe a context where 1,10-phenanthroline slowly, but strongly, activates MAPK while it impairs protein synthesis in a zincdependent manner, abolishing any feed-back loop. The induced-MAPK response was found to be strongly ultrasensitive. We argue that underlying this behaviour is a regulation motif akin to a feed-forward loop acting in vivo.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2013-01-30
Info
Issue
Section
Original Articles
Keywords:
MAPK, ultrasensitivity, feed-back, protein synthesis, regulation motif.
Statistics
  • Abstract views: 3168

  • PDF: 600
How to Cite
Beaujois, R., Riquet, F., Cailliau, K., Browaeys-Poly, E., Russo, C., Blossey, R., Vicogne, D., Marin, M., Lescuyer-Rousseau, A., Vilain, J.-P., & Bodart, J.-F. (2013). Ultrasensitive MAPK/Erk activation in absence of protein synthesis in Xenopus oocytes. MAP Kinase, 2(1), e2. https://doi.org/10.4081/mk.2013.e2