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Chemical composition and insecticidal activity Mentha piperita L. essential oil against the cowpea seed beetle Callosobruchus maculatus F. (Coleoptera: Bruchidae)

K. Saeidi, S. Mirfakhraie
  • K. Saeidi
    Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran, Islamic Republic of
  • S. Mirfakhraie
    Plant Protection Department, Faculty of Agriculture, Urmia University, Urmia, Iran, Islamic Republic of |


Antifeedant activity of plant extract from Mentha piperita was tested against the cowpea seed beetle, Callosobruchus maculatus. Dry ground plant was subjected to hydrodistillation using a Clevenger-type apparatus. The resulting oil contained menthone (28.9%), menthol (28.5%), and pulegone (6.9%) as the major compounds. LC50 of M. piperita essential oil was 25.70 μL/L air and mortality increased as the doses of essential oil increased. LT50 values were 3.29, 2.74 and 1.89 h at 100, 200 and 500 μL/L air, respectively. The results of persistence test of oil revealed that essential oil of M. piperita on C. maculatus adults was 5.44 days. It was clear from the repellency index that M. piperita essential oil has strong repellency at 90, 180 and 360 μL/L air concentration. The results showed that M. piperita essential oil has significantly affected the relative growth rate, relative consumption rate and feeding deterrence index of C. maculatus adults and it was concentration-dependent. The efficiency of conversion of ingested food has decreased while the concentrations are not statistically different at 5% level compared to the control group. It seems that essential oil can be used as low-risk insecticides.


essential oil, Mentha piperita, fumigant toxicity, repellency, persistence, nutritional indices, Callosobruchus maculatus

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Submitted: 2017-04-29 23:07:53
Published: 2017-12-31 00:00:00
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Copyright (c) 2017 K. Saeidi, S. Mirfakhraie

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