Original Articles
Vol. 13 No. 1 (2025)
https://doi.org/10.4081/hls.2025.13016
Explication of the mixture of Piper betle and Eugenia polyantha on oral bacteria
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 5 September 2024
Accepted: 2 December 2024
Published: 11 February 2025
Accepted: 2 December 2024
Published: 11 February 2025
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The aim of this study was to analyze the effectiveness of the mixtures of Eugenia polyantha and Piper betle leaves against oral bacteria. Four compositions of EP and PB were made and observed on tryptic soy agar, blood agar, MacConkey Agar (MCA), and Mannitol Salt Agar (MSA). Inhibition analysis was conducted at concentrations of 20%, 30%, 40%, 50%, and 60%. Our study results indicate that Staphylococcus aureus exhibited growth on BA, bacterial colonies were identified on MSA, and serological tests confirmed coagulation. A biochemical test conducted on a MCA sample revealed several species within the Enterobacteriaceae family, including Escherichia coli, Pseudomonas, Klebsiella, and Proteus. The highest level of inhibition was observed with the sixth toothbrush (K4). Significant differences in inhibition were noted among the various groups (K1, K2, K3, K4, and controls), with the diameter of inhibition for each combination yielding p<0.05. However, the differences in inhibition between the combinations themselves were not statistically significant, with p>0.05. Streptococcus sp, Klebsiella sp, Enterobacteriaceae sp (Coliform sp), Pseudomonas, and Staphylococcus aureus are the contaminant bacteria on used toothbrushes. The highest inhibition level and bactericidal properties were achieved by mixing 70% bay leaf (Eugenia polyantha) and 30% betel leaf infusion.
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Huang R, Li M, Gregory RL. Bacterial interactions in dental biofilm. Virulence 2011;2:435–44.
Tan HC, Cheung GSP, Chang JWW, et al. Enterococcus faecalis shields Porphyromonas gingivalis in dual-species biofilm in oxic condition. Microorganisms 2022;10:1–16.
Kumar S, Tadakamadla J, Johnson NW. Effect of toothbrushing frequency on incidence and increment of dental Caries: A Systematic Review and Meta-Analysis. J Dent Res 2016 ;95:1230–6.
Blaustein RA, Michelitsch L-M, Glawe AJ, et al. Toothbrush microbiomes feature a meeting ground for human oral and environmental microbiota. Microbiome 2021;9:32.
Frazelle MR, Munro CL. Toothbrush contamination: a review of the literature. Nurs Res Pract 2012;2012:1–6.
Raiyani CM, Arora R, Bhayya DP, et al. Assessment of microbial contamination on twice a day used toothbrush head after 1-month and 3 months: An in vitro study. J Nat Sci Biol Med 2015;6:44–8.
Nurjanah N, Herijuliant E, Putri M, Sukmasari S. Eugenia polyantha (Wight) infusion against oral microorganisms on toothbrushes. Scientific Dental J 2020;4:105.
Unahalekhaka A, Butpan P, Wongsaen R, et al. Contamination of antimicrobial-resistant bacteria on toothbrushes used with mechanically ventilated patients: A cross sectional study. Intensive Crit Care Nurs 2022;68:103120.
do Nascimento C, Trinca NN, Pita MS, Pedrazzi V. Genomic identification and quantification of microbial species adhering to toothbrush bristles after disinfection: A cross-over study. Arch Oral Biol 2015;60:1039–47.
Anand PJS, Athira S, Chandramohan S, et al. Comparison of efficacy of herbal disinfectants with chlorhexidine mouthwash on decontamination of toothbrushes: An experimental trial. J Int Soc Prev Community Dent 2016;6:22–7.
Niveda R, Kaarthikeyan G. Comparative evaluation of microbial contamination of neem infused toothbrush with regular toothbrush in periodontitis patients. 2019;12:1218–20.
Neal PR, Rippin JW. The efficacy of a toothbrush disinfectant spray--an in vitro study. J Dent 2003;31:153–7.
Konidala U, Nuvvula S, Mohapatra A, Nirmala SVSG. Efficacy of various disinfectants on microbially contaminated toothbrushes due to brushing. Contemp Clin Dent 2011;2:302–7.
Basman A, Peker I, Akca G, et al. Evaluation of toothbrush disinfection via different methods. Braz Oral Res 2016;30:1–6.
Aires A, Barreto AS, Semedo-Lemsaddek T. Antimicrobial effects of essential oils on oral microbiota biofilms: The toothbrush in vitro model. J Antibiot (Basel) 2020;10:1–16.
Shin AR, Nam SH. Antimicrobial effects of various methods for the disinfection of contaminated toothbrushes. Biomed Res 2018;29:2880–4.
Mavani V, Mahabala KY, Suman E, et al. Evaluation of effectiveness of home remedies for toothbrush decontamination using vinegar and vinegar with common salt. World J Dent 2018;9:19–23.
El Hamdaoui NEA, Knezevic M, Knezevic M, Vicente-Barrero MM. Cross section study and analysis of toothbrushes contamination and disinfection Study of 101 toothbrushes emloyed from the people of different ages. Head Neck Russian J 2020;8:45–51.
Saleh FR. The effect of using Dettol, salt and hot tap water in elimination of toothbrush contamination. Indian J Microbiol Res 2021;2:227–30.
Zhang A, Sun H, Wang X. Potentiating therapeutic effects by enhancing synergism based on active constituents from traditional medicine. Phytother Res 2014;28:526–33.
Guimarães R, Barros L, Carvalho AM, Ferreira ICFR. Infusions and decoctions of mixed herbs used in folk medicine: Synergism in antioxidant potential. Phytother Res 2011;25:1209–14.
Ischak NI, Botutihe DN. Preliminary study of clinical antidiabetic activity of Salam leaves (Eugenia polyantha) and Sambiloto leaves (Andrographis paniculata). IOP Conf Ser: Earth Environ Sci 2020;589:1–6.
Safriani N, Arpi N, Erfiza NM. Potency of Curry (Murayya koeniigi) and Salam (Eugenia polyantha) leaves as natural antioxidant sources. Pak J Nutr 2015;14:131–5.
Rohaeti E, Karunina F, Rafi M. FTIR-based fingerprinting and chemometrics for rapid Investigation of antioxidant activity from Syzygium polyanthum extracts. Indonesian J Chemistry 2020;21:128.
Nor MM. Phytochemicals screening, antioxidant activity and frying quality as affected by aqueous extract of Malaysian serai kayu (Eugenia polyantha). IMPACT: Int J Res App Nat Soc Sci 2015;3:81–8.
El Muna Haerussana AN, Widyastiwi W. Inhibitory test of Bay leaf (Eugenia polyantha) extract against Pseudomonas aeruginosa and Escherichia coli. J App Sci Eng Technol Educ 2022;4:223–8.
Aldhaher ZA, Merza WM, Almelan MF, et al. Effectiveness of bay leaves aqueous extract on Streptococcus mutans in comparision to chlorhexidine gluconate. J Pharm Biol Sci 2017;12:12–6.
Periyanayagam K, Jagadeesan M, Kavimani S, Vetriselvan T. Pharmacognostical and phyto-physicochemical profile of the leaves of Piper betle L. var Pachaikodi (Piperaceae) — Valuable assessment of its quality. Asian Pac J Trop Biomed 2012;2:S506–10.
Biswas P, Anand U, Saha SC, et al. Betelvine (Piper betle L.): A comprehensive insight into its ethnopharmacology, phytochemistry, and pharmacological, biomedical and therapeutic attributes. J Cell Mol Med 2022;26:3083–119.
Lubis RR, Marlisa, Wahyuni DD. Antibacterial activity of betle leaf (Piper betle l.) extract on inhibiting Staphylococcus aureus in conjunctivitis patient. Am J Clin Exp Immunol 2020;9:1–5.
Nayaka NMDMW, Sasadara MMV, Sanjaya DA, et al. Piper betle (L): Recent review of antibacterial and antifungal properties, safety profiles, and commercial applications. Molecules 2021;26:1–21.
Putri MH, Nurjanah N, Laela DS, Sukmasari S. Protective effects of mouthwash formulations of Syzygium polyantha (L.) and Piper betel (L.) on oral microbiota-induced gingivitis. Healthc Low-resour S 2023;12:11768.
Kateete DP, Kimani CN, Katabazi FA, et al. Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Ann Clin Microbiol Antimicrob 2010;9:23.
Jung B, Hoilat GJ. MacConkey Medium. StatPearls Publishing; 2022. Available from: https://www.ncbi.nlm.nih.gov/books/NBK557394/
Spellerberg B, Brandt C. Laboratory diagnosis of Streptococcus pyogenes (group A streptococci). Va FJSD, editor. Oklahoma City (OK): University of Oklahoma Health Sciences Center; University of Oklahoma Health Sciences Center; 2016. Available from: https://www.ncbi.nlm.nih.gov/books/NBK343617/
Putri MH, Julaeha E, Herijulianti E, Nurjanah N. Formulation of mouthwash for gingivitis from combination infusion of Salam leaves (Eugenia polyantha wight) and Betel leaf (Piper betle. L). Padjadjaran J Dent 2022;34:239–48.
O’Toole GA. Classic spotlight: Plate counting you can count on. J Bacteriol 2016;198:3127.
How to Cite
Explication of the mixture of Piper betle and Eugenia polyantha on oral bacteria. (2025). Healthcare in Low-Resource Settings, 13(1). https://doi.org/10.4081/hls.2025.13016
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