Cover Image

Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae

C.P. Narkhede, C.D. Patil, R.K. Suryawanshi, S.H. Koli, B.V. Mohite, S.V. Patil
  • C.P. Narkhede
    School of Life Sciences, North Maharashtra University, Maharashtra, India
  • C.D. Patil
    School of Life Sciences, North Maharashtra University, Maharashtra, India
  • R.K. Suryawanshi
    School of Life Sciences, North Maharashtra University, Maharashtra, India
  • S.H. Koli
    School of Life Sciences, North Maharashtra University, Maharashtra, India
  • B.V. Mohite
    School of Life Sciences, North Maharashtra University, Maharashtra, India
  • S.V. Patil
    School of Life Sciences, and North Maharashtra Microbial Culture Collection Centre, North Maharashtra University, Maharashtra, India | satish.patil7@gmail.com

Abstract

For effective vector control it is essential to formulate new preparations having multiple action against the vector pest. Developing combined formulation of biopesticide and chemical pesticide is one of the novel concept to fight against the vectors with new weapons; however, compatibility of biopesticide i.e. Bacillus thuringiensis (Bt) and chemical pesticide is a real hurdle. In this investigation, local isolate Bacillus thuringiensis SV2 (BtSV2) was tested for its compatibility with various available mosquito larvicides. Temephos was most compatible with BtSV2 than with other tested pesticides. These two compatible agents were tested for larvicidal potential. Our study revealed that the synergistic effect of both agents reduces LC50 value by 30.68 and 22.36% against the Ae. aegypti and An. stephensi, respectively. The larvicidal potential increased when compared to individual pesticides. It was also observed a biochemical change in larvae after the TBT (Temephos + Bacillus thuringiensis) combination treatment; it involves decreased level of alpha esterase, acetylcholine esterase and protein while level of beta esterase and acid phosphatase was unchanged and alkaline phosphatase activity was increased. Increased potential of combined formulation may be due to altered physiological condition.

Keywords

Combination; Bacillus thuringiensis (Bt); Temephos; Esterase; Protease; Larvicidal

Full Text:

PDF
HTML
Submitted: 2016-09-02 09:31:59
Published: 2017-04-21 10:18:49
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
416

Views:
PDF
205
HTML
297

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2017 C.P. Narkhede, C.D. Patil, R.K. Suryawanshi, S.H. Koli, B.V. Mohite, S.V. Patil

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185