Ozone Therapy intends to be the accessible and innovative leading edge interdisciplinary journal for the ozone-therapy practitioners worldwide, by publishing in different languages to be easier referred to and by establishing its own source of scientific information. Ozone Therapy seeks to publish peer-reviewed, original manuscripts dedicated to oxygen-ozone therapy; innovative observational works to open new therapeutic frontiers and study/verification scientific papers regarding known and registered applications; interdisciplinary papers devoted to other disciplines (e.g. Management, Finance, Healthcare) connected with the use of ozone in different fields. This journal does not apply the article processing charge to Authors as it is supported by institutional funds. Ozone Therapy is published under the auspices of the SIOOT (Italian Society for Oxygen-Ozone Therapy).
PAGEPress Scientific Publications, Pavia, Italy
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Use of oxygen-ozone therapy in the treatment of panniculopathy edematous fibro sclerotic: a clinical case
Oxygen-ozone therapy represents an effective therapeutic reality for panniculopathy, particularly in the edematous phase, as well as for the treatment of localized adiposity. Our case report is a 38 years-old patient with Edematous Fibrosclerotic Panniculopathy (EFP) diagnosed through physical examination. We compared the anthropometric measurements at the beginning of the treatment and after 18 sessions of oxygen-ozone therapy and it was seen that despite the study was conducted only on one patient, comparing our clinical case with the clinical cases of studies whose number was significant, we can say that EFP therapy with oxygen ozone produces satisfactory results both from a subjective point of view and from an objective point of view.
Indication of oxygen ozone therapy in the back pain of the sportsman with spongy edema type modic 1
Sport has contradictory effects on the lumbar spine, because while on the one hand it guarantees a strengthening of the “Muscular Core” of the trunk and increases its stability and elasticity, on the other hand it subjects the body to repeated stress and micro-trauma that would not occur in normal life and if prolonged over time cause damage to the structures, rather than a benefit. There are some conditions predisposing to lumbago in sportsmen and women that lead to the onset of the pathology determined mainly by functional overload of the spine. The rapid biomechanical movement produces a displacement of the pulpy nucleus which, if it fails to reposition itself, frequently causes micro lesions of the annulus with consequent degeneration of the disc in the medium term. In our study, only 20% of our athletes with back pain were found to have an anatomical cause, such as a herniated disc or vertebral stenosis, predominantly a discopathy related to Modic 1 type spongy edema. There is in fact a very strong correlation between the Modic type 1 sign, which is an indication of spongy tissue edema associated with trabecular fracture and back pain. One of the hypothetical causes of Modic type 1 bone edema has been attributed to the infection of bacteria such as: Proprionibacterium acnes, Corynebacterium propinquum and Staphylococcus, demonstrating the effectiveness of Modic Antibiotic Spinal Therapy (MAST) on the symptom of pain in patients with chronic lumbago.
Oxygen-ozone immunoceutical therapy in COVID-19 outbreak: facts and figures
The problems related to the approach to the outbreak of COVID-19 in the world require that all possible effective treatment options be explored. The clinical criterion of the researcher is not to refuse a priori, but to verify and evaluate the proposals that are made. Italian Society of Oxygen Ozone Therapy (SIOOT) proposed to the Italian ISS (Italian Institute of Health) to use oxygen ozone therapy (O2O3) in patients with COVID-19. (...)
Oxygen-ozone therapy: an extra weapon for the general practitioners and their patients
Oxygen-ozone therapy is a medical practice validated in the literature by numerous international clinical studies. It has indications in many diseases and complications, alone or in combination with conventional therapies. In this work, a number of international clinical studies have been evaluated and those most relevant to pathologies of daily interest to the general practitioner (GP) have been selected to assess their cost/benefit ratio. It has been concluded that GPs and their patients can derive significant benefits from this medical practice, and GPs are ideally placed to assess which patients should receive this treatment.
Treatment with oxygen-ozone therapy in hemodialysis patients
Peripheral arterial disease and secondary amyloidosis account for significant morbidity and mortality among uremic patients. Several papers demonstrated the beneficial effects of ozone therapy in the treatment of vascular and orthopaedic diseases. The aim of this paper is to study these effects among hemodialysed patients with severe complications. Seventy-five hemodialysed patients were studied. Sixty patients suffered from peripheral arterial occlusive disease. Twenty-three of them underwent previous unilateral lower limb amputation (Group 1). Fifteen subjects had remarkable joint pain due to a2-microglobulin deposition (Group 2). All patients were treated with analgesic therapy. The Italian Society for Oxygen-Ozone Therapy (SIOOT) protocols were applied: the patients received major O3 autohemotherapy, subcutaneous ozone infusion and body exposure to oxygen/ozone mixture by bagging. A questionnaire was submitted to the patients at the beginning and at the end of ozone therapy for the individual assessment of treatment efficacy. Complete healing of the ischemic ulcers was obtained in many patients. Eight-eight percent of the patients reported a subjective successful outcome after ozone therapy. Both Group 1 and Group 2 subjects reported a significant decrease in pain perception. The use of analgesic drugs was reduced in 78% of them and 72% got a normal sleep/wake cycle. An enhancement in physical activity was reported by 67% of the subjects. Ozone therapy improves hemodialysed patients’ quality of life reducing bone pain perception and attenuating clinical signs of peripheral vascular disease.
Rational approach, technique and selection criteria for the treatment of lumbar disc herniation with Regenerative Selective Disc therapy
Low back pain is one of the most common and important clinical, social, economic, and public health problems affecting the human population worldwide. The mechanism of radicular pain in the lumbar region is multifactorial but is likely due to mechanical and/or inflammatory factors. The natural history of disc herniation tends to be favourable. The Italian Society for Oxygen-Ozone Therapy (SIOOT) and the Italian Society for Spinal Surgery (SICV) guidelines recommend a conservative therapeutic approach. The biological action of medical ozone is still under investigation but some mechanisms of action have been proposed to explain its efficacy in disc herniation treatment: i) reduction of the inflammatory components; ii) hyper-oxygenation of the area of interest; iii) diminishing the size of the herniation; iv) stimulation of the repair process. The primary objective of this pilot study was to compare pain and function scores from patients before and after the treatment with Regenerative Selective Disc. The metrics that were established to define significant improvement were: i) improvement >1.8 on the Visual Analogue Scale (VAS) score; ii) improvement of 1 level or more on the modified Macnab criteria; iii) improvement >15% on the Oswestry Disability Index (ODI). Primary endpoints of this study were changes in the pretreatment and 1 month post treatment VAS, ODI and Macnab scores of the patients. After treatment results show that the patient population’s mean VAS, ODI and modified Macnab scores improved beyond the significant improvement scores. In fact, results showed that the mean patient’s population improvement scores were 2.9 for VAS, 22 for ODI and 1.4 for modified Macnab. Additionally, significant improvements were shown for 87% of the patients for VAS scale (>2.0), 80% of the patients for the ODI scale (>15 points) and 80% patients for the modified Macnab criteria (>1 point).
Anti-inflammatory effect of an eye drops solution based on liposomal ozonated oil in different corneal and anterior segment human diseases
The gaseous ozone can be effectively stabilized – for topical use – as ozonated oil, an organic analog of ozone, that has the same properties; the tolerability on the ocular surface has been further improved by means a specific formulation, developed for ophthalmic use, based on liposomal sunflower ozonated oil plus hypromellose (Ozodrop®, FB Vision, Ascoli Piceno, Italy), which is extremely biocompatible with the delicate ocular surface tissue. The use of ozone in certain eye anterior segment pathologies could be providential due to its anti-inflammatory and bactericidal activity, in addition to promoting tissue repair properties. Nowadays, the overuse of antibiotics in the treatment of infectious diseases, and the appearance of multidrug-resistant bacterial strains, has driven research towards the study of new resistance-free antimicrobial agents. In our experience, this new liposomal ozonated oil formulation was used to promote wound healing and treat some inflammatory and infectious pathologies of the anterior segment, such as vernal conjunctivities, granulomatous conjunctivities, persistent dystrophic corneal ulcer, i.e. diseases necessitating adequate anti-inflammatory and regenerative therapy. In these conditions, a topical dosage of one or two drops of collyrium every 4 h over 3-7 days is generally recommended, even though multiple and frequent instillations are often required to achieve clinical resolution. The use of ozone-based eye drops gave a faster resolution of the inflammatory and/or infectious process, healing of the wounded area, improvement of corneal deepithelization and resolution of the symptomatology (itching, soreness) and clinical sign. In conclusion, from our preliminary results, ozone-based eye drops represent a valid and suitable alternative therapy for the management of external ocular pathologies, both inflammatory and/or infective and traumatic.
SIOOT International: A call to unity All ozone practitioners belong to the same scientific space, worldwide
Copyright (c) 2019 Luigi Valdenassi, Marianno Franzini
Treatment experiences of gonarthrosis by intra-articular infiltration of ozonated autologous blood
Knee arthrosis is a degenerative process with inflammatory characteristics that develops in the articular environment, resulting in irreversible alterations that tend to impair its functionality. In this study, we subjected 136 patients to a new infiltrative therapy by means of autologous ozonated blood. The study yielded positive results on pain symptoms and functional recovery.
Association of oxygen-ozone therapy with high-power photodynamic therapy: a new protocol for the treatment of periodontopathies
In this study, we want to demonstrate that oxygen-ozone therapy together with laser therapy (high-power photodynamic therapy) leads to substantial and rapid benefits for periodontopathic patients.
Medication-related osteonecrosis of the jaw (MRONJ), an adverse reaction to medication, has been reported since 2002, at first in cancer patients with bone metastases treated intravenously with pamidronate or zoledronate, and later in patients taking oral bisphosphonate for the treatment of osteoporosis. Several cases of MRONJ have also been described in patients treated with antiangiogenic drugs with a so-called biological target (bevacizumab, sunitinib, sorafenib). Oxygen/ozone therapy for MRONJ treatment represents a possible and efficient alternative to the two main therapeutic approaches currently in use, antibiotic therapy and surgery. Since 2010, I have diagnosed 51 MRONJ cases at ASST Fatebenefratelli-Sacco. Subsequently and following the approval of the Ethics Committee (2015), patients with MRONJ level 1 and 2 have been included in an experimental protocol with oxygen/ozone therapy: Treatment of bisphosphonate-related osteonecrosis of the jaw with oxygen/ozone therapy and surgical debridement with piezoelectric scalpel. The study is currently running and up to now six out of fourteen patients healed perfectly. Among these six, four spontaneously or almost spontaneously have expelled the necrotic part without undergoing surgery. In three cases, there was only an improvement in symptoms. Five patients are currently being treated. Because of its antiseptic properties, oxygen ozone therapy reduced the effects of infection and inflammation, while the bio stimulant action of this therapy promoted the demarcation of bone sequestration and soft tissue healing.
H.A.R.O.T. Human assisted reproduction ozone therapy: the use of Oxygen-Ozone therapy as an adjunct in the therapy of couple sterility
The increase in couple sterility and the average increase in the age of women seeking pregnancy has placed the scientific community in front of the need to improve the quality of oocytes and the percentage of the embryonic implantation. The oxygen- ozone therapy seems to be able to help in this research thanks to its modes of action. A therapeutic protocol, identified with the acronym H.A.R.O.T (Human assisted reproduction ozone therapy) has been developed, the first results of which seem to be considerably encouraging in order to obtain a greater number and quality of oocytes and improve the percentage of embryonic implantation.
Copyright (c) 2019 Alfonso Maria Irollo, Maria Francesca Gangale, Gennaro Calabrese, Ezio Stortini, Sara Zanconi, Raffaele Aiello, Catella Criscuolo, Vincenzo Infante, Antonella Tartaglione, Simona Pizzinelli, Maria Laura De Simone
The use of intraoperative oxygen-ozone therapy to prevent capsular contracture in breast augmentation
Capsular contracture is the most frequent complication in breast augmentation. It has multifactorial aetiology but biofilm is suggested to have a central role in the capsular contracture pathogenesis. To sterilize the breast cavity and the implant after insertion we decided to use a mixture of oxygen and ozone, using ozone sterilizing properties with no subsequent contamination related to manipulation.
Copyright (c) 2018 Carlo Gasperoni, Paolo Gasperoni, M. Stella Malavenda
Determining the cytotoxic effect potential of ozonated hazelnut oil
Although the use of hazelnut oil is becoming ever more common, the ozonated form of this oil has never been obtained before. Ozonated vegetable oils are generally preferred in ozone therapy as they are easy to apply and obtain. In this study, the density, viscosity, peroxide and iodine values of hazelnut oil obtained by being treated with ozone in different periods at a flow rate of 7-8 were determined and compared with its commercial form, i.e. refined hazelnut oil. In order to determine whether ozonated hazelnut oil can be safely used, its cytotoxic effect potential was assessed by performing 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium assay on three different cell lines. For this purpose, the viability at five different concentrations of each sample was calculated after 48 and 72 hours of incubation in the H1299 lung cancer, human umbilical cord vein endothelial cells and A549 cell line. As a result, it was found that hazelnut oil applied to three different cell lines and in five different concentrations (100/50/25/12.5/6.25μg/mL) did not have any cytotoxic effects. Furthermore, it was also revealed that the samples did not have a toxic effect due to treatment with ozone in comparison to refined ozonated hazelnut oil. Therefore, this study has shown that ozonated hazelnut oil can be used as an alternative to refined hazelnut oil without exerting any toxic effects due to treatment with ozone. In light of this information, it was concluded that hazelnut oil can be safely used after treatment with ozone without having any cytotoxic effects and that it can be produced as a functional oil providing both the pharmacological and cosmetic effects of ozone.
Copyright (c) 2018 Sami Serhat Tonus, Sibel Bayıl Oğuzkan, Halil İbrahim Uğraş, İbrahim Halil Kılıç
The use of ozone as a driver for economic and environmental sustainable development
Sustainability, as a scientific concept, is multidisciplinary and very wide topic. Sustainable development bases its strategies not simply on the concept of growth, but rather on that of optimisation, around three main principles: ecosystem integrity, economic efficiency and social equity. The purpose of this paper, after an introduction about the concept of sustainability in general and as a strategy for the enterprises, is to discuss the potential of the use of ozone in different field, as a source of sustainable development, according to two of those principles, i.e. economic and environmental. In the first part, the concept of sustainability will be investigated in deep, with a managerial point of view, while the second part of the work will briefly analyse the current state of the arts of the uses of ozone, with also some practical examples. Finally, the discussion will take care of the reasons why the use of ozone, in several fields, could be considered as a driver for sustainability.
Ozone therapy is an effective therapy in chronic fatigue syndrome: result of an Italian study in 65 patients
The terms chronic fatigue syndrome and myalgic encephalomyelitis describe a complex physical illness characterized by debilitating fatigue, post-exertional malaise, pain, cognitive problems, sleep dysfunction and an array of other immune, neurological and autonomic symptoms. At the MEDE Clinic of Sacile, Italy, from February 2016 to December 2017, we have treated 65 patients, with auto hemo transfusion, according to the Scientific Society of Oxygen Ozone Therapy (SIOOT) protocols, twice a week for one month and twice a month as maintenance therapy. In conclusion, at our knowledge this is the largest study of patients with chronic fatigue syndrome treated with ozone therapy. Oxygen ozone therapy is an effective therapy in the treatment of chronic fatigue syndrome. However, more patients are needed and in particular a longer follow up is a necessary. In the meantime, ozone therapy seems a treatment that, also because without any side effect, is possible to be proposed to patients with chronic fatigue syndrome that are not obtaining sufficient results from available therapy.
Copyright (c) 2018 Umberto Tirelli, Cinzia Cirrito, Martina Pavanello
Oxygen-ozone therapy in dentistry: current applications and future prospects
Starting from 2012, the dental research community began to assess the benefits of oxygen-ozone on human metabolism, for its immune-stimulant and anti-hypoxic action, the increase in protein biosynthesis and activation of angiogenesis. We report the case of a 69-year-old patient, for whom oxygenozone therapy was used as a treatment that could reduce the bone resorption of the alveolar ridges, considering the reduced state of hypoxia resulting therefrom, the improved neoangiogenesis and the positive effect on bone metabolism. The results exceeded our greatest expectations, given that the measurements even showed an increase in bone peaks.
Ozone therapy in 40 patients with fibromyalgia: an effective therapy
Fibromyalgia is a chronic disorder with a very complex symptomatology. There is evidence that oxidative stress is increased in fibromyalgia, although it is not known whether this increase is involved as a causative factor in the development of the disease, or whether it is secondary to the patients’ unfit condition. Ozone therapy is thought to act by exerting a mild, transient, and controlled oxidative stress that promotes an up regulation of the antioxidant system and a modulation of the immune system. The objective of the present study was to get a preliminary evaluation of the potential effectiveness of ozone therapy in the management of fibromyalgia. At our knowledge, this is the largest study of patients with fibromyalgia treated with ozone therapy reported in the literature and it demonstrates that the ozone therapy is an effective treatment for fibromyalgia patients without significant side effects.
Effects of oxygen-ozone therapy in the Roth syndrome: a case report
Meralgia paresthetica (MP) is a disesthetic and/or anesthetic syndrome in the distribution of the lateral femoral cutaneous nerve. It is a compressive or traumatic mononeuropathy, characterized by burning pain and/or discomfort in the anterolateral thigh, without motor or muscle strength changes, with preserved reflexes. The authors report the case of a 52-year-old male with a history of low back pain presented from two-year sensory dysesthesias and paresthesias in the right anterolateral thigh, consistent with meralgia paresthetica; they also describe the first reported use of O2O3 therapy to relieve the intractable pain associated with MP.
Copyright (c) 2018 Alfredo Romeo, Carolina Baiano, Marianna Chierchia, Francesco Scandone
Microbiological aspects of ozone: bactericidal activity and antibiotic/antimicrobial resistance in bacterial strains treated with ozone
Antimicrobial resistance is one of the most complex global health challenges today. The world has long ignored warnings that antibiotics and other medicines are losing their effectiveness after decades of overuse and misuse in human medicine, animal health and agriculture. Common illnesses like pneumonia, postoperative infections, diarrhoeal and sexually transmitted diseases, as well as the world’s largest infectious disease killers – tuberculosis (TB), HIV and malaria – are increasingly becoming untreatable because of the emergence and spread of drug resistance. Worsening antimicrobial resistance could have serious public health, economic and social implications. The threat of antimicrobial resistance is also becoming a key consideration for programmes addressing maternal and child health, sexual and reproductive health, foodborne diseases, water and sanitation, and infection prevention and control. Although the 21st century is being shaped by technology and innovation, humans could soon find themselves in an era where simple infections once again kill millions every year. The past three years have seen unprecedented global political momentum to address antimicrobial resistance: in 2015, governments adopted a global action plan at the World Health Assembly and in 2016 passed a political declaration at the United Nations General Assembly. Antimicrobial resistance has made it onto the agendas of the G7 and G20 groups and is a core component of the Global Health Security Agenda. WHO is working closely with the Food and Agriculture Organization of the United Nations and the World Organization for Animal Health in leading global efforts against antimicrobial resistance and ensuring that the necessary momentum is consolidated and sustained. These efforts are guided by an ad-hoc interagency coordination group established in 2017. A global development and stewardship framework to combat antimicrobial resistance is being drafted to support the development of new antimicrobial medicines, diagnostics, vaccines and other tools. One of the gravest global concerns about antimicrobial resistance currently is that antibiotic resistance has emerged in so many pathogens, including TB. In 2016, at the high-level meeting of the UN General Assembly on antimicrobial resistance, Heads of State directed an unprecedented level of attention to curbing the spread of infections that are resistant to antimicrobial medicines. They reaffirmed their commitment to stopping the misuse of antimicrobial medicines in human health, animal health and agriculture, and recognized the need for stronger systems to monitor drug-resistant infections and the amounts of antimicrobials used in humans and animals. In the wake of the increasing global awareness of the need for new antibiotics, Member States highlighted market failures, and called for new incentives for investment in research and development of new, effective and affordable medicines, rapid diagnostic tests, and other important therapies to replace those that are losing their effectiveness. In response to this and in line with the Global Action Plan on Antimicrobial Resistance to support the identification of pathogens of greatest concern, WHO developed a priority list of antibiotic resistant bacteria to underpin renewed efforts for the research and development of new antibiotics. The only possible defence against the threat of antimicrobial resistance and the (very real) possibility of a post-antibiotic era is a global and coordinated effort by all stakeholders to support other important therapies such as the Oxygen-Ozone (O2O3) therapy. As a result, the scope and focus of the work underlying this dissertation was to study the application of O2O3 therapy towards several resistant bacteria. Moreover, we evaluated three different framework for gut bacteria, skin and soft tissue infections and mucosal infections.
Copyright (c) 2018 Giuseppe Giuliani, Giovanni Ricevuti, Antonio Galoforo, Marianno Franzini
Outpatient evaluation of oxidative stress in subjects undergoing systemic oxygen-ozone therapy
Oxygen-ozone therapy improves the quality of life in patients affected by various diseases. The purpose of this study was to verify and eventually quantify the production of free radicals generated by oxidative stress induced by the ozonated auto-hemoinfusion in subjects subjected to this practice and eventually evaluate and naturally quantify the plasma antioxidant power of the subjects under examination. All subjects showed a significant improvement in the oxidative balance already at the first check, which was maintained (albeit with a minimal non-significant loss) at the end of the study. The results of the study have made it possible to verify that systemic ozonated auto-hemoinfusion not only does not involve further oxidative stress but even exerts an important antioxidant effect, through the scavenger effect of free radicals.
Copyright (c) 2019 Fortunato Loprete, Francesco Vaiano, Luigi Valdenassi
Oxygen-ozone therapy in arthroplasty infections
The prevention of surgical site infections is a priority within the objectives of the National Health System. The negative consequences of postoperative infections (morbidity, duration of hospitalization, mortality) are particularly serious events for the individual patient and for the community, given both the welfare resources needed to treat them, and the continued growth of the phenomenon of antibiotic resistance. In a global health context in which evidence-based medicine has established itself, all medical interventions must be reconsidered in the light of what can be inferred from the literature evaluated according to strict methodological criteria, all the more so when the evidence is available and unanimously shared. The future objective will be to introduce in the national guidelines a protocol involving the use of ozone therapy alongside antibiotic therapy, both in the prevention and treatment of infections, to avoid the emergence of antibiotic resistance and thus reduce the expenditure of the national health system.
Copyright (c) 2019 Alessandra Vittoria De Lisi, Marianno Franzini, Giovanni Ricevuti
Use of ozone in water, agriculture and zootechnics: relationships between dysbiosis and mental disorders
Ozone (O3) is a bluish-coloured gas with a characteristic odour that forms in the layers of the atmosphere, near electric shocks, sparks or lightning; the extremely high voltages reached during thunderstorms produce ozone from oxygen. The particular fresh, clean odour, the smell of spring rain is the result of the ozone reproduced by nature. Ozone comes from the Greek word ozein, which means to sense the odour of. Ozone is an essential gas for life on Earth, allowing the absorption of ultraviolet light emanating from the Sun; in fact, the ozone layer in the stratosphere protects against the harmful action of UV-B ultraviolet rays. The gas, not being stable over the long term, is therefore not produced in cylinders; it can currently be prepared through special, certified and authorised devices, which use small electric discharges to convert the oxygen into ozone. It is a molecule formed by three oxygen atoms (O3), with a negative electric charge. It has a short half-life, and will therefore decay after a certain time back to its original form: oxygen. Essentially ozone is nothing but oxygen (O2) with an extra oxygen atom, which has a high electrical charge. Ozone works according to the principle of oxidation. The oxidation mechanism follows two paths: i) Direct: contact of the molecule with the contaminant; ii) Indirect: the ozone decomposes into hydroxyl radicals, more powerful but short-lived. Both reactions occur simultaneously. When the static charged ozone molecule (O3) comes into contact with something capable of oxidising, the ozone molecule’s charge flows directly over it. This happens because ozone is very unstable and tends to change back into its original form (O2). Ozone can oxidise with all kinds of materials, but also with odours and microorganisms such as bacteria, viruses and fungi. The supplemental oxygen atom is released from the ozone molecule and binds to the other material. In the end, only the pure and stable oxygen molecule remains. Ozone is one of the strongest oxidation techniques available for oxidising solutes. The supplemental/added oxygen atom will bind (=oxidation) in a second to each component that comes into contact with ozone. It is used for a wide range of purification processes. It can be employed for disinfection in municipal wastewater and in drinking water treatment plants. However, ozone is increasingly used in the industrial sector. In the food industry, for example, it is used for disinfection, and in the textile and paper industry it is used to oxidise wastewater. The main benefit of ozone is its clean nature, because it only oxidises the materials, barely forming any by-products. Since ozone has a strong characteristic distinctive odour, even very low concentrations can be quickly perceived. This generally makes it safe to work with. Since Chlorine is still the best-known oxidising and disinfectant agent, ozone is often compared with chlorine. Unlike chlorine, antibiotics or various chlorine derivatives that have no effect, ozone acts on viruses and spores. In its sterilising action, ozone directly attacks bacteria by inducing a catalytic oxidation process on the mass of bacterial proteins, unlike chlorine which acts only through specific enzymatic poisoning of vital centres, a process which requires a longer time interval and sensitive quantity for its diffusion inside the cytoplasm. Regarding the virucidal action, it is interesting to keep in mind that with a residual ozone rate of 0.6 ppm (parts per million) and with a contact time of 2 minutes, the percentage of inactivation for bacteria and viruses present in the disinfection liquid is total. Ozone’s oxidising power is 120 times greater than that of chlorine.
Copyright (c) 2019 Giuseppe Vitali, Luigi Valdenassi
Oxygen-ozone therapy in meningoencephalitis and chronic fatigue syndrome. Treatment in the field of competitive sports: case report
Our study was born from the observation of a clinical case of a boy who arrived in the Emergency Room of our hospital for persistent hyperpyrexia, headache and prolonged emetic episodes and complaining of objective photophobia and dizziness. The patient underwent haematochemical tests, chemical-physical examination of Chronic Fatigue Syndrome (CFS), negative for bacteria, negative for Neisseria Meningitidis, Escherichia Coli 121, Haemophilus Influenzae, Stafilococcus Pneumoniae, Stafilococcus Agalactiae and with slightly positive reaction to Pandy’s test; he was subjected to neurological examination, to Nuclear Magnetic Resonance of the brain and encephalic trunk with contrast agent which resulted negative, and to EEG that showed a slightly slowed-down brain electrical activity, in right occipital region, and frontal irritative abnormalities. Given these clinical and instrumental investigations, an acute meningoencephalitis was diagnosed. During his hospitalization, the patient was treated with intravenous antibiotic therapy and intravenous antiviral therapy for 12 days. At discharge, in the absence of specific therapy, and considering the protraction of the cephalic, dizzying, asthenic and myalgic symptoms and in relation to hematochemical and serological tests (positive for antibodies to Herpes 1 IgG), Epstein Barr Virus antibodies (positive for Viral Capsid Antigen IgG and IgGE BNA, for Extractable Nuclear Antigen and IgG Cytomegalovirus) he was diagnosed a Post-infectious CFS. The patient was treated with Oxygen Ozone Rectal Insufflative Therapy on a bi-weekly basis for 4 weeks, associated with Micetrin, a dietary supplement with sweetener based on Vitamin C, Shitake, Reishi, Maitake, Cordyceps, Magnesium and SOD, continued the treatment on a weekly basis for a further 4 weeks until the complete remission of the symptoms of asthenic, neurological and clinical parameters.
Copyright (c) 2019 Luca Morelli, Simona Carla Bramani, Federico Carlo Morelli
New frontiers in rehabilitation. Oxygen-ozone therapy and neuronal plasticity in the treatment of Parkinson's symptoms
Oxygen-ozone therapy is effective for both activation of the cerebral microcirculation and increasing energy production by neurons, as recent studies have shown. As well as this, it has been shown to have a proven anti-inflammatory and antispasmodic action, improve microcirculation, increase the production of cellular energy, activate the mitochondrial antioxidant system and facilitate the elimination of cell catabolites. It also seems that oxygen-ozone therapy improves and prolong the effects of drugs such as Levodopa, Selegiline and Bromocriptine and antioxidants, therefore these activities could help us understand the improvements recorded in Parkinson’s patients treated with oxygen-ozone therapy. The aim of our work was to carry out a postural, stabilometric and symptomatological assessment of the effects of oxygen-ozone therapy by using rectal insufflation in patients who undertook the intense motor activity of non-contact boxing. The results showed a significant reduction in the myofunctional, postural and stabilometric symptoms of Parkinsonian patients who practiced non-contact boxing activities, implemented though systemic oxygen-ozone therapy with insufflation.
Copyright (c) 2018 Luca Morelli, Simona Carla Bramani, Alessandra Guarino
Ozone therapy in painful lipodystrophies. A preliminary study
The study focuses on lipomas, which are quite common, occur in one person out of every thousand and generally arise individually, although about 20% of those who suffer from them may have multiple cases. Ozone oxygen therapy has been shown to be effective and free of adverse side effects in all treated patients, and there are no contraindications to the use of the therapy. In most patients, the lipoma shrinks completely. The oxygen-ozone procedure is a scientifically approved and highly effective treatment for lipodystrophy.
Copyright (c) 2018 Olga Cardoso, Pierluigi Rossi, Antonio Galoforo, Guido Collodo
Oxygen-ozone therapy in prevention and rehabilitation of myocardium infarct
In 1991, Prof. Lettieri (Naples, Federico II University) using Great Ozonated Blood Auto Infusion (GAE) treated patients having acute myocardium infarct, he had good results concerning pain and prognosis. Oxygen-ozone therapy is protective for ischemic cardiopathy. In 1996, oxygen-ozone therapy was used in relapse myocardium infarct prevention, showing an important factor against infarct relapse. Ozone has an important anti-inflammatory and rheological action, even if it is a strong oxidant. Paradoxically ozone activates cells antioxidant system, activates cells enzymatic function against free radicals and increases DNA antioxidant enzyme transcription. Ozone also activates redoxine system, reduce pro inflammatory cytokines II 1β, TNFX, modulate NFKB system, reduce platelet aggregation and stimulate different growth factor release. Because of the above skills, oxygen-ozone therapy helps to prevent ischemic cardiopathy and post infarcted rehabilitation. A mixture of ozone and oxygen administration has been evaluated on myocardium damaged tissues after an experimental ischemic event and myocardium reperfusion. Infarct damage can be counteract using pre- treatment with oxygen-ozone gaseous mixture. All our cardiopath patients treated with GAE have had an emo dynamic benefit, left ventricle ejection fraction improvement. Patients also improved aritmologic aspect with atrial fibrillation disappearance, enhance or disappearance of different comorbidity that often cardiopath/ischemic patients suffer.
Copyright (c) 2018 Sergio Pandolfi, Angelo Zammitti, Marianno Franzini, Vincenzo Simonetti, William Liboni, Luigi Valdenassi, Filippo Molinari
Pharmacoeconomic analysis of ozone therapy supported by agent based process simulation and data mining
Pharmacoeconomics is the set of methods applied to the economic evaluation of pharmacological treatments. Its goal is to determine the best possible health outcome with the available resources by comparing costs (inputs) and the consequences/benefits (outcomes) of one or more alternative choices. Ozone therapy can be considered as a powerful alternative to traditional methods in the management and treatment of several diseases, like, e.g. rheumatoid arthritis, lumbago, disc hernia, knee and hip disease, fibromyalgia, stroke outcomes, neurodegenerative syndromes and more. It is thus a very interesting topic to compare the costs and outcomes of ozone based treatments with those coming from other alternative methods, with the perspective of pharmacoeconomics. Several methods have been proposed for pharmacoeconomical analysis, including statistical and simulation tools. The purpose of this research paper is to review some of the simulation tools that could be used in this field and in particular to propose a hybrid simulation method, defined as agent based process simulation, which considers not only the involved costs, but also other non-monetary outcomes. In the same vein, a data mining set of techniques is proposed to collect data and to feed the simulation model.
Copyright (c) 2018 Marco Remondino, Luigi Valdenassi, Marianno Franzini
Oxygen-ozone and α-lipoic acid in the treatment of peripheral neuropathies conflicting therapies or synergistic association?
The report in question is divided into four parts: i) neuropathies: pathophysiology notes; ii) oxidative stress: conceptual points useful for this report; iii) lipoic acid: general characteristics with particular mention of the characteristics of the product that can influence the action of the ozone; iv) oxygen-ozone therapy and lipoic acid: conflict or synergistic association?
Treatment of piriformis syndrome with oxygen-ozone therapy
Piriformis syndrome is a pain-causing disease of the sciatalgic type, also called false sciatalgia. Indeed, it is possible that a suffering of this muscle, caused by different etiologies, is responsible for a more or less pronounced sciatalgic pain. All this in the absence of overtly pathological problems such as a disc hernia, a canalized stenosis or a mass in a ischio-crural region. We dealt with this problem in a group of patients who had been submitted to various therapies in the immediately preceding periods: pharmacology, tecartherapy and manual physiotherapy. After these treatments, these subjects had the same painful symptoms without any improvement. Therefore, they were treated with oxygen ozone by infiltration. Evaluation according to the visual analog scale, baropodometric examination and clinical trials of Freiberg and Page and Nagle were performed before, in the middle, and at the end of the treatment. The results were very encouraging, showing a noticeable improvement until the disappearance of symptoms.
Copyright (c) 2018 Luigi Valdenassi, Debora Bellardi