Scientific Articles
Vol. 25 No. 1 (2016)
https://doi.org/10.4081/microscopie.2016.5271

Epifluorescence microscopy imaging of phytoplasmas in embedded leaf tissues using DAPI and SYTO13 fluorochromes

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Received: 15 May 2015
Published: 8 April 2016
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DAPI, epifluorescence microscopy, phytoplasmas, stolbur, SYTO13®, tomato.
plant science, plant pathology, phytoplasma

Authors

S.V. Buxa
Justus Liebig University, Giessen, Germany.
L. Pagliari
University of Udine, Italy. image/svg+xml
R. Musetti
rita.musetti@uniud.it
University of Udine, Italy. image/svg+xml
The use of DNA-specific dyes, i.e. DAPI, is extensively reported for phytoplasma detection in fresh plant materials. However,fluorescence-based microscopy and imaging of fresh tissues often evidences technical limitations which are more significant in infected tissues, because phenolic and other defense-related compounds accumulate in the cell wall and in the vacuole making difficult sample preparation. In this paper we describe a method based on the use of epifluorescence microscopy and the DNA probes DAPI and SYTO13® for phytoplasma visualization in resin-embedded plant tissues. The method allows detection of phytoplasmas and it is recommended for tissues that are recalcitrant to conventional imaging.

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Supporting Agencies

DAAD, Germany, University of Udine
R. Musetti, University of Udine
Department of Agricultural and Environmental Sciences

How to Cite



Epifluorescence microscopy imaging of phytoplasmas in embedded leaf tissues using DAPI and SYTO13 fluorochromes. (2016). Microscopie, 25(1), 49-56. https://doi.org/10.4081/microscopie.2016.5271

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