QUERCETIN AND RUTIN: EFFECTS OF TWO FLAVONOIDS ON INDUCED OXIDATIVE STRESS IN HUMAN EJACULATED SPERM

Submitted: 20 August 2012
Accepted: 20 August 2012
Published: 20 August 2012
Abstract Views: 2666
PDF: 1309
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Quercetin (Q) and rutin (R) are two natural flavonoids with antioxidant properties. We evaluated the toxicity of Q and R at 20μM, 30μM, 50μM, 100μM, 200μM, 400μM in swim up selected human sperm. The antioxidant activity (Q and R 20μM and 30μM) was tested on lipid peroxidation (LPO) induced by tert-butylhydroperoxide in human sperm. LPO was evaluated using the C11-BODIPY581/591 probe and sperm structural damages were assessed by transmission electron mi- croscopy in samples incubated with and without Q and R. A significant dose dependent toxic effect was observed for both compounds on sperm viability (Q and R: r= -0.98 P<0.001), on sperm progressive motility (Q: r=-0.98, R: r=-0.97; P<0.001), and on non progressive motility (Q; r=-0.58, P<0.01; R: r=-0.50, P<0.05). Both flavonoids, used at 20μM and 30μM, showed antioxidant properties on LPO induced in human sperm and a general protective effect against ultrastructural damages of LPO. In conclusion, we observed that Q exhibited a little higher toxicity than R; on the other hand R is little low protective on induced LPO. Our preliminary results demonstrated the scavenger properties of these flavonoids in vitro on human sperm.

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Mazzi, L., Geminiani, M., Collodel, G., Iacoponi, F., Martini, S., Bonechi, C., Rossi, C., & Moretti, E. (2012). QUERCETIN AND RUTIN: EFFECTS OF TWO FLAVONOIDS ON INDUCED OXIDATIVE STRESS IN HUMAN EJACULATED SPERM. Journal of the Siena Academy of Sciences, 3(1), 22–26. https://doi.org/10.4081/jsas.2011.564