LOCALIZATION OF HUMANIN IN HUMAN, RABBIT AND MOUSE SPERM


Submitted: 10 January 2012
Accepted: 10 January 2012
Published: 10 January 2012
Abstract Views: 885
PDF: 1251
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Authors

  • M. Geminiani Department of Biomedical Sciences, Applied Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
  • E. Moretti Department of Biomedical Sciences, Applied Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
  • V. Giannerini Department of Biomedical Sciences, Applied Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
  • L. Rossini Siena Biotech, University of Siena, Italy.
  • R. Cardinali Department of Applied Biology, Animal Science Section, University of Perugia, Italy.
  • L. Trabalzini Department of Molecular Biology, University of Siena, Italy.
  • T. Renieri Department of Biomedical Sciences, Applied Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
  • G. Collodel Department of Biomedical Sciences, Applied Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
Humanin (HN) is a peptide showing neuroprotective properties against damage associated with Alzheimer’s disease. In male reproductive system HN is expressed in human and rat testis. This preliminary study aimed to localize HN by immunocytochemistry (ICC) directly in human, mouse and rabbit sperm. Semen samples were processed for the ICC analyses using two different rabbit polyclonal anti-HN antibodies. Western blotting analyses were performed using fresh human semen samples. Normal human, rabbit and mouse sperm, showed HN labeling in sub-acrosomal and mid-piece regions, whereas human abnormally shaped sperm were stained also at acrosomal level and particularly in the flagellum. Western blotting analysis revealed the presence of HN in analyzed human semen samples. HN was localized directly on human, rabbit and mouse spermatozoa. In human samples, we demonstrated a different localization pattern in normally shaped sperm compared to abnormal sperm probably due to a protective effect of HN in pathological sperm.

Geminiani, M., Moretti, E., Giannerini, V., Rossini, L., Cardinali, R., Trabalzini, L., Renieri, T., & Collodel, G. (2012). LOCALIZATION OF HUMANIN IN HUMAN, RABBIT AND MOUSE SPERM. Journal of the Siena Academy of Sciences, 1(1), 64–67. https://doi.org/10.4081/jsas.2009.340

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