“051 | Optimization of a combined spectral and imaging flow cytometry protocol integrated with AI for rapid human microbiota screening: Arianna Aquilini-Mummolo1|2, Francesca D’Ascanio1|2|3, Giulia Colasante1|2, Domenico De Bellis1|2, Pasquale Simeone4, Sabino Porro1|2, Tamer Esmail1|2, Ayesha Younas1|2, Luana D’Onofrio1|2, Isabel Vallejo Bermúdez5|6, Camilla Cichella2, Mariagiulia Filoso1|2, Chiara Porro4, Paola Lanuti1|2. | 1Department of Medicine and Aging Sciences, University “G d’Annunzio”, Chieti-Pescara, Chieti; 2Center for Advanced Studies and Technology CAST, University “G d’Annunzio”, Chieti-Pescara; 3Department of Humanities, Law and Economics, ‘Leonardo da Vinci’ University, Torrevecchia Teatina, Italy; 4Department of Clinical and Experimental Medicine, University of Foggia, Italy; 5Molecular Immunology Group CTS208, Department of Cell Biology, Physiology and Immunology, University of Cordoba, Cordoba, Spain; 6Immunology and Allergy Group GC01, Maimonides Biomedical Research Institute of Cordoba IMIBIC, University of Cordoba, Reina Sofia University Hospital, Cordoba, Spain”., J Biol Res, vol. 99, no. s1, Mar. 2026, doi: 10.4081/jbr.2026.15303.