Differential regulation of plasma proteins between members of a family with homozygous HbE and HbEβ-thalassemia

  • Suchismita Halder Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata, India.
  • Tridip Chatterjee Institute of Genetic Medicine and Genomic Science, Kolkata, India.
  • Amit Chakravarty Institute of Genetic Medicine and Genomic Science, Kolkata, India.
  • Sudipa Chakravarty Institute of Genetic Medicine and Genomic Science, Kolkata, India.
  • Abhijit Chakrabarti | abhijit.chakrabarti@saha.ac.in Crstallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India.

Abstract

In this report we’ve compared the plasma protein profiles of 4 individuals in a family. Father and the younger son both are hemoglobin (Hb) Eβ-thalassemic {Cod 26 (G-A)/IVS 1- 5 (G-C)}, but the father never requires transfusion, whereas the younger son requires monthly blood transfusion. Mother and the elder son are HbEE {Cod 26 (G-A)/Cod 26 (GA)} without any history of transfusion. Proteomic study was done on the plasma fraction of the blood following ammonium sulphate precipitation. Proteins were separated by 2D-gel electrophoresis, expression of proteins compared by densitometry and proteins identified by tandem MALDI mass spectrometry. Proteins responsible in hemolysis, hypercoagulation and hemoglobin scavenging have shown differential regulation, establishing the relation between the differences in the levels of plasma proteins with the progression of the disease phenotype, manifested in the extent of transfusion dependence of the patient.

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Author Biographies

Suchismita Halder, Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata

Senior Research fellow

Tridip Chatterjee, Institute of Genetic Medicine and Genomic Science, Kolkata

Senior Research Fellow

Amit Chakravarty, Institute of Genetic Medicine and Genomic Science, Kolkata

professor

Sudipa Chakravarty, Institute of Genetic Medicine and Genomic Science, Kolkata

professor

Published
2014-09-29
Keywords:
HbE-β thalassemia, blood transfusion, differential proteomics.
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How to Cite
Halder, S., Chatterjee, T., Chakravarty, A., Chakravarty, S., & Chakrabarti, A. (2014). Differential regulation of plasma proteins between members of a family with homozygous HbE and HbEβ-thalassemia. Thalassemia Reports, 4(2). https://doi.org/10.4081/thal.2014.1837