@article{Havaei_Rezaei_Havaei_Namvar_2017, title={Detection of enterotoxins and genotyping of Staphylococcus aureus strains isolated from Isfahan Educational Hospital, Iran}, volume={32}, url={https://www.pagepressjournals.org/mm/article/view/6965}, DOI={10.4081/mm.2017.6965}, abstractNote={<em>Background and aims:</em> <em>Staphylococcus aureus</em> is known as one of the most important nosocomial pathogens, which may lead to several infections. The aim of this study was determining the enterotoxins A, C, and TSST-1 and molecular characterization of <em>S.</em> <em>aureus</em> strains with PFGE and MLST typing methods. <br /><em>Materials and methods:</em> In the present study during the sixmonths sampling, fifty <em>S.</em> <em>aureus</em> strains were isolated from patients admitted to Al-Zahra university hospital. Antimicrobial susceptibility testing, Multiplex PCR for detection of enterotoxin A, C and TSST-1, pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular typing. <br /><em>Results</em>: In antibiogram the highest and lowest percentage of resistance was belonged to tetracycline and rifampin respectively. Multiplex PCR indicated that 30% of the strains harbored sea and 34% harbored sec genes. However, only 4% of our collected isolates had tsst gene. In PFGE method analysis on all <em>S. aureus</em> strains, a total of 19 different patterns were identified. Nine various sequence types in 27 selected <em>S. aureus</em> isolates were identified by MLST. <br /><em>Conclusions</em>: Present study indicates a possible higher variability among our <em>S. aureus</em> strains by two different molecular typing methods; nevertheless four main common types (CT1, CT7, CT9, and CT11) with at least one toxin genes were determined.}, number={3}, journal={Microbiologia Medica}, author={Havaei, Seyed Asghar and Rezaei, Nahid and Havaei, Roholla and Namvar, Amirmorteza Ebrahimzadeh}, year={2017}, month={Oct.} }