Identification using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry: Preliminar Data

  • Elisa Gobbato | office@pagepress.org
  • Vesselina Kroumova
  • Marcella Perone
  • Paola Ruzza
  • Giacomo Fortina

Abstract

Bacterial identification is usually allowed analyzing the phenotypic and biochemical topics of microorganisms. The traditional methods of identification require about 18-24 hours and are expensive. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) allows the identification of bacteria in few minutes. We consider 459 bacterial strains that were identified both with MALDI TOF MS and VITEK-2. The percentage of concordance in every group of bacteria was more than 99%, at genus level were more than 94% and at species level was more than 92%. These preliminary data show that MALDI-TOF MS allows a rapid and precise microorganism identification.

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Published
2011-06-30
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Issue
Section
Original Articles
Keywords:
Mass spectrometry, Rapid identification, Bacteria
Statistics
  • Abstract views: 1148

  • PDF: 971
How to Cite
Gobbato, E., Kroumova, V., Perone, M., Ruzza, P., & Fortina, G. (2011). Identification using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry: Preliminar Data. Microbiologia Medica, 26(2). https://doi.org/10.4081/mm.2011.2360

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