Detection of C. difficile common antigen: comparison of methods

Maria Giuliana Brunelli; Sabrina Tamburelli; Gian Lorenzo Molinari; Stefano Andreoni

doi:10.4081/mm.2011.2356

Detection of C. difficile common antigen: comparison of methods

https://doi.org/10.4081/mm.2011.2356

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Vol. 26 No. 3 (2011)

Submitted: 13 February 2014
Accepted: 13 February 2014

Published: 30 September 2011

C. difficile, Common antigen (GDH), Antibiotic-associated pseudomembranous colitis

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In order to identify and define a diagnostic algorithm for the diagnosis of C. difficile infection, a comparative study was carried out at the Microbiology Laboratory of “Maggiore della Carità” Hospital in Novara.We compared the system currently in use in the laboratory “TECHLAB C.difficile Quik Chek Complete (Inverness Medical, USA)”, an immunoenzymatic assay for the simultaneous detection of C. difficile common antigen (GDH) and Toxins A&B, with the new ImmunoCard Clostridium difficile GDH (Meridian Bioscience, USA), which identifies the C. difficile Common Antigen GDH. The results proved identical between the two assays: of 100 samples, 82 resulted negative with both tests, 18 were GDH positive with both tests. Out of the 18 GDH positives, 9 resulted positive for the Toxins portion of the TechLab test.

Brunelli, M. G., Tamburelli, S., Molinari, G. L., & Andreoni, S. (2011). Detection of C. difficile common antigen: comparison of methods. Microbiologia Medica, 26(3). https://doi.org/10.4081/mm.2011.2356