Typing of Enterococcus spp. strains isolated from patients with infective endocarditis by an automated repetitive-sequence-based PCR system

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Giuseppe Ivan Russello *
Claudio Farina
Annibale Raglio
Veronica Ravasio
on behalf of the Italian Infective Endocarditis Study Group (SEI)
(*) Corresponding Author:
Giuseppe Ivan Russello | farina.claudio@sancarlo.mi.it

Abstract

The use of rep-PCR was studied to characterize 32 isolates of Enterococcus spp. from Italian patients with infective endocarditis and determine specific banding patterns associated with antimicrobial resistance phenotypes. The results obtained with rep-PCR were analyzed with respect to the antimicrobial profile by a MIC evaluation (E-test) of all strains tested. The automated rep-PCR technique resulted an easy method for the characterization of Enterococcus spp. It confirmed the eterogenicity of enterococcal strains involved in infective endocarditis. Furthermore results of antimicrobial susceptibility revealed that rep-PCR can not be used as diagnostic tool to characterize and identify difference between resistant and susceptible Enterococcus spp. strains.

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