Molecular epidemiology of C. pneumoniae infections
AbstractIntroduction. Chlamydophila pneumoniae (C. pneumoniae) is one of the most common respiratory pathogen, with an incidence of infection varying from 6% to 20%. The present study aimed to assess the incidence of C. pneumoniae infections in patients with acute respiratory diseases using a RealTime PCR (RT-PCR) method. Methods. In the period January 2007-December 2008 279 biological samples coming from patients (190 males and 89 females) with acute respiratory infections was collected and tested. Samples have been extracted using NucliSens easyMag Biomerieu according to manufacturer’s instructions and amplified by LightCycler Real-Time PCR Roche for the detection of C. pneumoniae DNA. Results. Data analysis revealed a higher prevalence of C. pneumoniae infections in male patients (7.9%) than in females (5.6%). In addition, it is interesting to note that the incidence of C. pneumoniae infection was higher 28.6% in the period February - April. Conclusions. The results obtained in this study confirm the utility of molecular techniques in laboratory diagnosis and epidemiological investigations of respiratory infection caused by C. pneumoniae. RT-PCR have proved to be a rapid and a reliable technique to monitor and treat opportunely C. pneumoniae infections to avoid short and medium/long term complications.
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Copyright (c) 2010 Alisa Shurdhi, Federica Delucca, Francesca Savegnago, Maria Di Paolo, Manuela Donati
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