Sepsis: a more faster report from blood culture


Submitted: 14 February 2014
Accepted: 14 February 2014
Published: 30 September 2010
Abstract Views: 734
PDF: 797
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Introduction: Sepsis is a leading cause of morbidity and mortality. Several studies demonstrated that the earliness of the intervention therapy, including antimicrobial treatment active on the specific pathogen, is associated with a reduction of mortality. In order to permit the use of a quicker appropriate treatment in respect to using the conventional method,we evaluated both the accuracy of strain identification and in vitro antimicrobial susceptibilities directly from the haemocultural bottles. Methods:We examined 112 samples of positive blood cultures through traditional technique of subculture, identification and susceptibility testing, and a diagnostic alternative method in the following way: 5-6 ml from positive blood cultures (BACTEC 9640) were transferred into a test tube fitted with a sterile gel separator and centrifuged at 3200 rpm for 15 minutes. After the removal of supernatant, the microbial pellet was suspended in a saline buffer to obtain an 0.5 McFarland inoculum; this turbidity was needed to set up the direct identification and the susceptibility testing (VITEK 2). Results: The correlation between the two procedures has demonstrated a correlation of 98% for the strains identification; the correlation was of 100% for MRSA and ESBL recognition. Conclusions: The results showed that the use of the direct test for both Gram positive and Gram negative bacteria can give a complete report regarding the identification and susceptibility testing within 24 hours from when the sample becomes positive instead of 48h required using the conventional method.The procedure has demonstrated a high reliability both in terms of strains identification and of antibioticsusceptibility. We therefore can suggest, in the diagnostic of sepsis, the introduction of direct method in normal laboratory practice.

Ledonne, R., Cavalcanti, P., Filia, M. A., Perugini, D., Gaccione, C., Pingitore, P., & Giraldi, C. (2010). Sepsis: a more faster report from blood culture. Microbiologia Medica, 25(3). https://doi.org/10.4081/mm.2010.2431

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