Valuation of a new Cromogenic Agar Medium for the detection of ESBL producing Enterobacteriaceae

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Carlo Mancini
Daniela Danieli
Adrio Penni
Marta Mingione
Fabrizio Tosto
Gianluca Puggioni
Paola Varesi
Alessandra Giordano *
(*) Corresponding Author:
Alessandra Giordano | alessandra.giordano@uniroma1.it

Abstract

Escherichia coli producing ESBL are a major problem in the many different hospitals worldwide causing outbreaks as well as sporadic infections. A novel chromogenic agar medium (ChromID ESBL bioMèrieux, Marcy L’Etoile, France) was tested directly on a total of 162 clinical samples and plated in parallel on to common media including Mac Conkey agar (bioMèrieux, Marcy L’Etoile, France). Colonies presenting a different morphologic aspect and growing on media were identified and tested for susceptibility by VITEK 2 using GN-cards and AST-N013 cards (bioMérieux, Marcy L’Etoile, France) Confirmation of ESBL-producing isolates was performed by combinated double disk. We are not find errors.The chromID ESBL appears as an excellent medium for the screening and presumptive identification of ESBLproducing Enterobacteriaceae directly from clinical samples. Further studies with a wider range of clinical specimens are required to confirm this utility.

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