Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

  • Boris Lègba Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Victorien Dougnon | victorien88@hotmail.com Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Angèle Ahoyo Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Jerrold Agbankpè Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Gildas Hounmanou Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Alidah Aniambossou Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Edna Hounsa Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Kafayath Fabiyi Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Affousssath Amadou Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Phénix Assogba Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Honoré Bankolé Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Jacques Dougnon Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.
  • Lamine Baba-Moussa Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin.

Abstract

Objectives. This study aims to evaluate the antibacterial and chemical properties of some medicinal plants used in the fight against enteropathogens in Benin.
Methods. Aqueous and ethanolic extracts of Senna siamea, Uvaria chamae, Lantana camara and Phyllantus amarus were tested on 10 bacterial strains. Well diffusion technique, coupled with the microdilution determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CMB) was used for antibacterial testing. The larval cytotoxicity was evaluated by using Artemia salina crustacean larvae. flavonoids and polyphenols were also assayed by the method using aluminum trichloride (AlCl3) and the method using the folin-Ciocalteu reagent, respectively.
Results. The results of the study revealed that extracts had an effective antibacterial activity at 100 mg/mL, with MIC between 100 and 25 mg/mL and CMB between 100 and 50 mg/mL. The inhibition diameters of the extracts varied between 7.5 and 21 mm. The ethanolic extract of Phyllantus amarus leaves showed the best antibacterial activity. None of the extracts tested was found to be cytotoxic at the dose of 20 mg/mL. The aqueous Uvaria chamae root extract has the highest polyphenol content (231.896552±0.27586207 in μg EAG/100 mg extract), whereas the aqueous leaf extract of Uvaria chamae is the richest in flavonoids (41.061082 0.43180737 in μg ER/100 mg of extract).
Conclusions. These interesting results can be used in the development of improved traditional medicines against enteropathogens.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2018-02-27
Info
Issue
Section
Original Articles
Supporting Agencies
The World Academy of Science for the Advancement of Science in Developing Countries (TWAS) and the United Nation Educational, Scientific and Cultural Organization (UNESCO)
Keywords:
Enteropathogens, salmonellosis in Benin, beninese pharmacopoiea.
Statistics
  • Abstract views: 935

  • PDF: 239
  • HTML: 377
How to Cite
Lègba, B., Dougnon, V., Ahoyo, A., Agbankpè, J., Hounmanou, G., Aniambossou, A., Hounsa, E., Fabiyi, K., Amadou, A., Assogba, P., Bankolé, H., Dougnon, J., & Baba-Moussa, L. (2018). Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants. Microbiologia Medica, 32(4). https://doi.org/10.4081/mm.2017.6998