Cover Image

Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

Boris Lègba, Victorien Dougnon, Angèle Ahoyo, Jerrold Agbankpè, Gildas Hounmanou, Alidah Aniambossou, Edna Hounsa, Kafayath Fabiyi, Affousssath Amadou, Phénix Assogba, Honoré Bankolé, Jacques Dougnon, Lamine Baba-Moussa
  • Boris Lègba
    Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Victorien Dougnon
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin | victorien88@hotmail.com
  • Angèle Ahoyo
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Jerrold Agbankpè
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Gildas Hounmanou
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Alidah Aniambossou
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Edna Hounsa
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Kafayath Fabiyi
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Affousssath Amadou
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Phénix Assogba
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Honoré Bankolé
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Jacques Dougnon
    Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
  • Lamine Baba-Moussa
    Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin

Abstract

Objectives. This study aims to evaluate the antibacterial and chemical properties of some medicinal plants used in the fight against enteropathogens in Benin.
Methods. Aqueous and ethanolic extracts of Senna siamea, Uvaria chamae, Lantana camara and Phyllantus amarus were tested on 10 bacterial strains. Well diffusion technique, coupled with the microdilution determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CMB) was used for antibacterial testing. The larval cytotoxicity was evaluated by using Artemia salina crustacean larvae. flavonoids and polyphenols were also assayed by the method using aluminum trichloride (AlCl3) and the method using the folin-Ciocalteu reagent, respectively.
Results. The results of the study revealed that extracts had an effective antibacterial activity at 100 mg/mL, with MIC between 100 and 25 mg/mL and CMB between 100 and 50 mg/mL. The inhibition diameters of the extracts varied between 7.5 and 21 mm. The ethanolic extract of Phyllantus amarus leaves showed the best antibacterial activity. None of the extracts tested was found to be cytotoxic at the dose of 20 mg/mL. The aqueous Uvaria chamae root extract has the highest polyphenol content (231.896552±0.27586207 in μg EAG/100 mg extract), whereas the aqueous leaf extract of Uvaria chamae is the richest in flavonoids (41.061082 0.43180737 in μg ER/100 mg of extract).
Conclusions. These interesting results can be used in the development of improved traditional medicines against enteropathogens.

Keywords

Enteropathogens, salmonellosis in Benin, beninese pharmacopoiea.

Full Text:

PDF
HTML
Submitted: 2017-08-16 14:30:32
Published: 2018-02-27 09:30:07
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
207

Views:
PDF
13
HTML
40

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2017 Boris LEGBA, Victorien DOUGNON, Angèle AHOYO, Gildas HOUNMANOU, Alidah ANIAMBOSSOU, Edna HOUNSA, Jerrold AGBANKPE, Honoré BANKOLE, Jacques DOUGNON, Lamine BABA-MOUSSA

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2018     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185     •     Privacy