Culturomics: bacterial species isolated in 3 healthy donors for faecal microbiota transplantation in Clostridium difficile infection

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Luca Masucci *
Gianluca Quaranta
Domenico Nagel
Sandra Primus
Lucio Romano
Rosalia Graffeo
Gianluca Ianiro
Antonio Gasbarrini
Giovanni Cammarota
Maurizio Sanguinetti
(*) Corresponding Author:
Luca Masucci | luca.masucci@policlinicogemelli.it

Abstract

Background. Clostridium difficile infections are the main cause of nosocomial acquired diarrhea, because of prolonged antibiotic regimens. In the last years, mortality has increased due to recurrent infections caused by metronidazole and vancomicin resistant hypervirulent C. difficile strain 027. Faecal Microbiota Transplantation (FMT) is an infusion of faecal material obtained from healthy donors. This procedure reduces mortality in recurrent C. difficile infections (CDI). In this study we identified bacterial species obtained from donors’ stool samples using culturomics.
Materials and methods. Three donors were selected for FMT in three recipients affected by CDI. Faces obtained for FMT underwent culturomics, applying 17 different culture methods.
Results and conclusions. Several different bacteria were isolated from donors. In two donors 4 potentially pathogenic bacteria were isolated; this suggests the use of culturomics for donors’ screening or for selection of bacteria to infuse.

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