Phenotypic classification of multidrug-resistant Klebsiella through MALDI-TOF mass spectrometry

  • Anna Di Taranto | aditaranto@ospedaliriunitifoggia.it Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
  • Rosella De Nittis Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
  • Marco Fagioni Bruker Daltonics s.r.l., Macerata, Italy.
  • Tiziana Rollo Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
  • Paola Cella Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.
  • Raffaele Antonetti Department of Clinical Pathology, Analysis Laboratory II, Ospedali Riuniti of Foggia University Hospital, Italy.

Abstract

Background. Resistance to carbapenems in Klebsiella pneumoniae infections could compromise therapy efficiency in hospitals. In Italy, the percentage of K. pmeumoniae resistant to carbapenems blood-isolated is 35%.
Materials and Methods. Seventy-three multidrug-resistant K. pneumoniae clinical isolates coming from Foggia (Italy) have been phenotypically classified through matrix assisted laser desorption/ionization-time of flight mass spectrometry between February and May 2014 in order to locate an eventual infectious outbreak.
Conclusions. Classification of microbial isolates is essential to this purpose and starts from phenotypic methodologies to the most complex genotypic methods. Among these, mass spectrometry utilization appears to be a simple, useful and quick instrument in a clinical microbiology laboratory.

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Published
2015-06-18
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Original Articles
Keywords:
Klebsiella pneumoniae, KPC, MALDI-TOF MS
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How to Cite
Di Taranto, A., De Nittis, R., Fagioni, M., Rollo, T., Cella, P., & Antonetti, R. (2015). Phenotypic classification of multidrug-resistant Klebsiella through MALDI-TOF mass spectrometry. Microbiologia Medica, 30(1). https://doi.org/10.4081/mm.2015.5015