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Evaluation of LIAISON® C. difficile glutamate dehydrogenase and LIAISON® C. difficile toxin A and B in Copan FecalSwabTM samples in a three-step algorithm for the diagnosis of Clostridium difficile infection

Massimo Oggioni, Alessandra Bielli, Alice Nava, Daniela Adele Pia Campisi
  • Alessandra Bielli
    Chemical Clinical Analysis and Microbiology, Niguarda Ca’ Granda Hospital, Milan, Italy
  • Alice Nava
    Chemical Clinical Analysis and Microbiology, Niguarda Ca’ Granda Hospital, Milan, Italy
  • Daniela Adele Pia Campisi
    Chemical Clinical Analysis and Microbiology, Niguarda Ca’ Granda Hospital, Milan, Italy

Abstract

The presumptive laboratory diagnosis of Clostridium difficile infection is achieved by the means of the detection of a common antigen (glutamate dehydrogenase, GDH) in stool, then confirming the positives either by the detection of toxins A and B or by a molecular test for the detection of pathogenicity locus, encoding for the two toxins and for the binary toxin. A fully automated chemiluminescence system for the GDH antigen (LIAISON® C. difficile GDH) and for the detection of toxins A and B (LIAISON® C. difficile Toxin A and B) (DiaSorin, Gerenzano, Italy) allows for the performance of these tests on large numbers of samples in a short time, ensuring the traceability of the data.

Keywords

C. difficile, GDH, toxin A&B, chemiluminescence, 3 step algorithm, molecular, CDAD, immunochromatography

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Submitted: 2015-01-12 15:08:28
Published: 2015-06-18 00:00:00
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Copyright (c) 2015 Massimo Oggioni, Alessandra Bielli, Alice Nava, Daniela Adele Pia Campisi

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