La reazione polimerasica a catena (PCR) nella diagnosi delle infezioni genitali da Papillomavirus umano (HPV)

Rosanna Masciari; Ignazio Vasapollo; Maria Gabriella Lepore; Rosangela De Grazia; Vincenzo Rondinelli; Caterina Filia

doi:10.4081/mm.2004.2998

La reazione polimerasica a catena (PCR) nella diagnosi delle infezioni genitali da Papillomavirus umano (HPV)

https://doi.org/10.4081/mm.2004.2998

PDF

Vol. 19 No. 4 (2004)

Submitted: 25 February 2014
Accepted: 25 February 2014

Published: 31 December 2004

Polymerase Chain Reaction, HPV screening, HPV typing, p53 typing

Abstract Views: 767

PDF: 947

Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

In a three year time (2001-2003) we evaluated the use of HPV testing and typing in 520 cytologically abnormal women (322 LSIL, 198 HSIL), in 25 squamous and 18 adeno invasive cancers. HPV testing was performed by polymerase chain reaction (PCR) using L1 consensus primers; HPV typing was performed using specific primers for low and high risk types. The prevalence of high risk HPV was: LSIL 41%; HSIL 74%; squamous invasive cancers 96% and adeno 100%. HPV 16 is the most represented type in all lesions. We also evaluated the usefulness of p53 typing in the early identification of women at risk for cervical cancer.

Masciari, R., Vasapollo, I., Lepore, M. G., De Grazia, R., Rondinelli, V., & Filia, C. (2004). La reazione polimerasica a catena (PCR) nella diagnosi delle infezioni genitali da Papillomavirus umano (HPV). Microbiologia Medica, 19(4). https://doi.org/10.4081/mm.2004.2998