Utilizzo di nuovi test immunologici nella diagnostica dell’infezione da M. tuberculosis

  • Ilaria Sauzullo | ilariasauzullo@libero.it
  • Fabio Mengoni
  • Miriam Lichtner
  • Raffaella Rossi
  • Donatella Vincenti
  • Stefania Carrara
  • Delia Goletti
  • Enrico Girardi
  • Claudio Maria Mastroianni
  • Vincenzo Vullo

Abstract

New in vitro test, the Interferon-γ assay, has now emerged for diagnosis of latent tuberculosis infection (LTBI). Newer assays based on the immune response to ESAT-6 and CFP-10 antigens from the RD1 of M. tuberculosis may have advantages over TST, in terms of higher specificity, better correlation with exposure to M. tuberculosis and less cross-reactivity due to BCG vaccination and non-TB mycobacterial infection.Two commercially available tests have been approved for the diagnosis of TB infection, the QuantiFERON® TB-Gold and the T Spot.TB. These assays are based on overlapping peptides of ESAT-6 and CFP-10; however, they do not discriminate between A-TB and LTBI. Differently, using multiepitopic peptides from ESAT-6 and CFP-10 proteins selected by quantitative implemented HLA peptide-binding motifs analysis, it has bee shown a positive response to RD1 selected peptides only in patients with A-TB.Aim of this study is to evaluate the test TB-Gold as a tool to identify TB infection in a population enrolled with suspicious active TB. Agreement between TST and Interferon-γ assay (80%), rather then sensivity and specifity was reported. Moreover the assay based on RD1 selected peptides was used to evaluate whether it would allow a discrimination between active TB and LTBI. Our study suggest, that TB-Gold is useful tool to identify TB infection in a population enrolled with suspicious active TB. Moreover the assay based on RD1 selected peptides is a useful tool to discriminate between active TB and LTBI with a positive predictive value of 100% and a negative predictive value of 76%.

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Published
2006-12-31
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Short Communications
Keywords:
M. tuberculosis, TB-Gold, ESAT-6, CFP-10, IFN-g.
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How to Cite
Sauzullo, I., Mengoni, F., Lichtner, M., Rossi, R., Vincenti, D., Carrara, S., Goletti, D., Girardi, E., Mastroianni, C., & Vullo, V. (2006). Utilizzo di nuovi test immunologici nella diagnostica dell’infezione da M. tuberculosis. Microbiologia Medica, 21(4). https://doi.org/10.4081/mm.2006.2917

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