Usefulness of MALDI-TOF mass spectrometry in epidemiological control of etiologic agents of infection

  • Roberto Degl’Innocenti Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Tamara Brunelli | tbrunelli@usl4.toscana.it Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Lorenzo Pellegrini Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Daniele Ferri Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Elisa Rovinati Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Michele Rispoli Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Antonella Conti Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.
  • Patrizia Casprini Laboratorio Analisi Chimico Cliniche, Ospedale Misericordia e Dolce, ASL4, Prato, Italy.

Abstract

Introduction: The use of the MALDI-TOF mass spectrometry in the routine of microbiological diagnostics has revolutionized procedures and response times of bacteriology.The use of this technique aims to epidemiological investigations in a hospital environment and represents a further significant opportunity, quickly feasible and extremely economical. Methods: By means of the MALDI-TOF-MS Vitek2 (MS Vitek2) mass spectrometer, accompanied by the AgnosTec-SARAMIS (bioMérieux, France) software, were analyzed the spectra of 149 bacterial isolates (139 Staphylococcus aureus and 10 Staphylococcus epidermidis) obtained from cultures of 148 patients (141 inpatients and 7 outpatients). Clinical isolates were stored at a temperature of -20°C.The isolates were then thawed and immediately cultured on agar blood medium. The colonies were subjected to analysis by MS Vitek on the day after sowing. The spectra obtained were analyzed and compared using the software AgnosTec-SARAMIS, which allowed the construction of a dendrogram. Results and conclusions: The evaluation of the data collected suggests that mass spectrometry could be an useful tool in epidemiological surveys. Speed of analysis and low costs make the MS Vitek2 an usable tool by many microbiology laboratories.

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Published
2013-08-31
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Original Articles
Keywords:
mass-spectrometry, epidemiology, Staphylococcus epidermidis, Staphylococcus aureus
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How to Cite
Degl’Innocenti, R., Brunelli, T., Pellegrini, L., Ferri, D., Rovinati, E., Rispoli, M., Conti, A., & Casprini, P. (2013). Usefulness of MALDI-TOF mass spectrometry in epidemiological control of etiologic agents of infection. Microbiologia Medica, 28(2). https://doi.org/10.4081/mm.2013.2251