Electron tomography in the Scanning Electron Microscope for the investigation of biological and inorganic samples

Maurizio Donarelli; Matteo Ferroni; Luca Masini; Vittorio Morandi; Andrea Migliori; Luca Ortolani; Andrea Sanzogni; Alberto Signoroni

doi:10.4081/microscopie.2018.7370

Authors

Maurizio Donarelli
maurizio.donarelli@unibs.it
University of Brescia, Department of Information Engineering, Italy.
Matteo Ferroni University of Brescia, Department of Information Engineering CNR-Microelectronic and Microsystems Institute, Italy.
Luca Masini CNR-Microelectronic and Microsystems Institute, Italy.
Vittorio Morandi CNR-Microelectronic and Microsystems Institute, Italy.
Andrea Migliori CNR-Microelectronic and Microsystems Institute, Italy.
Luca Ortolani CNR-Microelectronic and Microsystems Institute, Italy.
Andrea Sanzogni University of Brescia, Department of Information Engineering, Italy.
Alberto Signoroni University of Brescia, Department of Information Engineering, Italy.

This paper reports on the implementation of electron tomography in the Scanning Electron Microscope, describing its application in both physical and biological sciences. The experimental set up for tomography is described with emphasis on the scanning-transmission imaging modality, which allows one to record a series of projective images of a thin specimen. The computation of the three-dimensional arrangement of the constituents in graphene-ZnO nanorods and in collagen fibrils in dermal tissue is discussed. In addition, we show how the implementation of a compressed sensing approach can be useful to preserve nanometric resolution and overcome the limitations of the experimental strategy.

Donarelli, M., Ferroni, M., Masini, L., Morandi, V., Migliori, A., Ortolani, L., Sanzogni, A., & Signoroni, A. (2018). Electron tomography in the Scanning Electron Microscope for the investigation of biological and inorganic samples. Microscopie, 29(1). https://doi.org/10.4081/microscopie.2018.7370

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