QUERCETIN AND RUTIN: EFFECTS OF TWO FLAVONOIDS ON INDUCED OXIDATIVE STRESS IN HUMAN EJACULATED SPERM


Submitted: 20 August 2012
Accepted: 20 August 2012
Published: 20 August 2012
Abstract Views: 2615
PDF: 1238
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

  • L. Mazzi Department of Biomedical Sciences, Applied Biology Section, University of Siena, Italy.
  • M. Geminiani Department of Biomedical Sciences, Applied Biology Section, University of Siena, Italy.
  • G. Collodel Department of Biomedical Sciences, Applied Biology Section; Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
  • F. Iacoponi Department of Biomedical Sciences, Applied Biology Section, University of Siena, Italy.
  • S. Martini Department of Pharmaceutical and Applied Chemistry, University of Siena; Centre for Colloid and Surface Science (CSGI), University of Florence, Italy.
  • C. Bonechi Department of Pharmaceutical and Applied Chemistry, University of Siena; Centre for Colloid and Surface Science (CSGI), University of Florence, Italy.
  • C. Rossi Department of Pharmaceutical and Applied Chemistry, University of Siena; Centre for Colloid and Surface Science (CSGI), University of Florence, Italy.
  • E. Moretti Department of Biomedical Sciences, Applied Biology Section; Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
Quercetin (Q) and rutin (R) are two natural flavonoids with antioxidant properties. We evaluated the toxicity of Q and R at 20μM, 30μM, 50μM, 100μM, 200μM, 400μM in swim up selected human sperm. The antioxidant activity (Q and R 20μM and 30μM) was tested on lipid peroxidation (LPO) induced by tert-butylhydroperoxide in human sperm. LPO was evaluated using the C11-BODIPY581/591 probe and sperm structural damages were assessed by transmission electron mi- croscopy in samples incubated with and without Q and R. A significant dose dependent toxic effect was observed for both compounds on sperm viability (Q and R: r= -0.98 P<0.001), on sperm progressive motility (Q: r=-0.98, R: r=-0.97; P<0.001), and on non progressive motility (Q; r=-0.58, P<0.01; R: r=-0.50, P<0.05). Both flavonoids, used at 20μM and 30μM, showed antioxidant properties on LPO induced in human sperm and a general protective effect against ultrastructural damages of LPO. In conclusion, we observed that Q exhibited a little higher toxicity than R; on the other hand R is little low protective on induced LPO. Our preliminary results demonstrated the scavenger properties of these flavonoids in vitro on human sperm.

Mazzi, L., Geminiani, M., Collodel, G., Iacoponi, F., Martini, S., Bonechi, C., Rossi, C., & Moretti, E. (2012). QUERCETIN AND RUTIN: EFFECTS OF TWO FLAVONOIDS ON INDUCED OXIDATIVE STRESS IN HUMAN EJACULATED SPERM. Journal of the Siena Academy of Sciences, 3(1), 22–26. https://doi.org/10.4081/jsas.2011.564

Downloads

Download data is not yet available.

Citations