TY - JOUR AU - Soldano, S. AU - Montagna, P. AU - Brizzolara, R. AU - Villaggio, B. AU - Ferrone, C. AU - Parodi, A. AU - Cutolo, M. PY - 2011/01/30 Y2 - 2024/03/29 TI - Effects of bosentan on collagen type I synthesis on in vitro culture of scleroderma skin fibroblasts JF - Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale JA - J Biol Res VL - 84 IS - 1 SE - Abstract Book DO - 10.4081/jbr.2011.4687 UR - https://www.pagepressjournals.org/jbr/article/view/4687 SP - AB - <p>The present study evaluated the effects of a non-selective endothelin (ET<sub>A/B</sub>) receptors antagonist, on collagen type I (COLI) synthesis on <em>in vitro</em> culture of scleroderma (SSc) skin fibroblasts (Fb). Fb were obtained from skin biopsies of 6 female SSc patients (mean age 64. 1<span class="st">±</span>6 years), after informed consent and Ethical Committee Approval. Cells were treated with endothelin-I [ET-I, 100nM] for 24 and 48 hrs, pre-treated for I hr with ET<sub>A/B</sub> receptors antagonist [10nM] alone or followed by ET-I for 24 and 48 hrs. Untreated Fb were used as controls. Immunocytochemistry and western blot analysis were performed to evaluate COLI synthesis. ET-I increased COLI synthesis both at 24 and 48 hrs when compared to controls. ET<sub>A/B</sub> receptor antagonost blocks the increased COLI synthesis ET-I-mediated both at 24 and 48 hrs vs. ET-I. Results showed that ET-I receptors blockage by ET<sub>A/B</sub> receptors antagonist might prevent the excessive synthesis of COLI, supporting its positive action in the management of skin fibrosis.</p> ER -