Effect of cadmium on anion exchange capability through Band 3 protein in human erythrocytes


Submitted: November 27, 2017
Accepted: February 13, 2018
Published: May 15, 2018
Abstract Views: 948
PDF: 428
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Authors

  • Rossana Morabito Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
  • Alessia Remigante Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
  • Benedetta Arcuri Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
  • Angela Marino Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
  • Marco Giammanco Department of Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy.
  • Giuseppina La Spada Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
  • Angela Marino Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy. http://orcid.org/0000-0002-6827-7327
The efficiency of Band 3 protein, mediating HCO3-/Cl- exchange across erythrocytes membrane, is reduced by oxidative stress. The aim of the present study was to verify whether Band 3 protein efficiency is compromised by treatment with Cadmium (Cd2+), an extremely toxic heavy metal known to interfere with antioxidant enzymes, energy metabolism, gene expression and cell membranes. To this end, the rate constant for SO4= uptake through Band 3 protein (accounting for velocity of anion exchange) was measured along with membrane –SH groups, Malonyldialdehyde (MDA) and Band 3 protein expression levels in Cd2+ -treated human erythrocytes (300 µM, 1 mM). Our results show that Cd2+ reduced the rate constant for SO4= uptake, with a significant increase in MDA levels at both concentrations and with a reduction in –SH groups observed after 1 mM Cd2+ treatment, whereas Band 3 protein expression levels were unchanged in both conditions. In conclusion: i) Cd2+ reduces Band 3 protein efficiency via different mechanisms depending on metal concentration and with unchanged expression levels; ii) the assessment of Band 3 protein anion exchange capability is a good tool to assay the impact of heavy metals on cell homeostasis and, possibly, useful for diagnosis and monitoring of devalopment of Cd2+ toxicity-related pathologies.

Morabito, R., Remigante, A., Arcuri, B., Marino, A., Giammanco, M., La Spada, G., & Marino, A. (2018). Effect of cadmium on anion exchange capability through Band 3 protein in human erythrocytes. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 91(1). https://doi.org/10.4081/jbr.2018.7203

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