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The peculiar behavior of dnaA, gyrA and gyrB temperature-sensitive mutants and their Hfr derivatives made recA, rnhA or both mutants

Erika Coppo, Anna Marchese, Eugenio Agenore Debbia
  • Erika Coppo
    C.A. Romanzi Section of Microbiology, Department of Surgical Sciences and Integrated Diagnostic (DISC), University of Genoa, Italy
  • Anna Marchese
    C.A. Romanzi Section of Microbiology, Department of Surgical Sciences and Integrated Diagnostic (DISC), University of Genoa; Microbiological Unit, San Martino Polyclinic Hospital, Genoa, Italy
  • Eugenio Agenore Debbia
    C.A. Romanzi Section of Microbiology, Department of Surgical Sciences and Integrated Diagnostic (DISC), University of Genoa, Italy | agenore44@gmail.com

Abstract

The role of the recA and rnhA mutations on the growth of different thermo-sensitive mutants at the non permissive temperature was studied. The growth of Hfr strains selected by integrative suppression in a dnaA(Ts) mutant was found strongly dependent on the RecA protein. This latter gene product was also essential for gyrA(Ts) and gyrB(Ts), rnhA double mutants for growing at 43°C. This RecA+ dependent cell multiplication was due to the fact that all the strains studied initiated their DNA synthesis from site(s) different from the normal gene oriC. This phenomenon causes in the cell a non-stop production of the bacterial genome with concomitant disorders in the bacterial division process. It has been suggested that when the growth of the microorganism is driven by an extra-chromosomal genetic element it behaves like a transformed tumor cell. Thus the bacterial model may present many advantages for studying the mechanisms by which cells lose the control of their division process because of an infecting foreign genetic element integrated in their chromosome.

Keywords

Hfr; DNA gyrase mutants; recA susceptibility; DNA synthesis with and without CM

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Submitted: 2017-06-29 16:03:24
Published: 2018-02-12 15:29:27
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Copyright (c) 2018 Eugenio Agenore Debbia, Erika Coppo, Anna Marchese

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