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Inhibition study of proinflammatory factor with ethanolic extract of propolis on innate immunity from diabetes mellitus mice model

Bambang Pristiwanto, Sutiman B. Sumitro, Muhammad S. Djati, Aris Soewondo, Hideo Tsuboi, Muhaimin Rifa’i
  • Bambang Pristiwanto
    Biology Master Program, Brawijaya University, Malang, Indonesia
  • Sutiman B. Sumitro
    Biology Department, Brawijaya University, Malang, Indonesia
  • Muhammad S. Djati
    Biology Department, Brawijaya University, Malang, Indonesia
  • Aris Soewondo
    Biology Department, Brawijaya University, Malang, Indonesia
  • Hideo Tsuboi
    Unit of Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai-shi, Aichi, Japan
  • Muhaimin Rifa’i
    Biology Department, Brawijaya University, Malang, Indonesia |


Health becomes an important topic today. One current problem was how to treat the effects of metabolic diseases, such as diabetes mellitus (DM). Thus, this study used an ethanolic extract of propolis (EEP), to test their ability as the supplement in the diabetes treatment to reduce inflammation, through proinflammatory factor response, especially nuclear factor κB (NF-κB). The streptozotocin- induced diabetes mellitus (SID) mice model was used, and expression of an proinflammatory factor was analyzed in their innate immunity cells with 3 doses of EEP, i.e. 50 mg/kg body weight, 100 mg/kg body weight, and 200 mg/kg body weight. Treatment of EEP in SID with three doses treatment decrease the number of macrophages with NF-κB expression significantly with DM control group. The results of B cells with NF-κB expression showed that EEP treatment in SID could decrease in dose 1 and dose 3, but not in dose 2. Proinflammatory cytokines expression of macrophage, especially Tumor Necrosis Factor-α and Interferon-γ, with EEP treatment in SID could decrease in three doses. This study suggests that EEP could reduce inflammation by inhibiting the development of NF-κB in innate immunity cells.


B cell; diabetes mellitus; macrophage; Nuclear Factor-κB; proinflammatory cytokines

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Submitted: 2017-03-31 08:10:47
Published: 2018-02-12 14:39:41
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