Susceptibility to patterns of ciprofloxacin among nalidixic acid-resistant Salmonella isolates collected in Banepa, Nepal from enteric fever patients

  • Girija Roka Department of Microbiology, National College, Kathmandu, Nepal.
  • Subash Pandaya Department of Pharmacy, North South University, Dhaka, Bangladesh.
  • Md. Reyad-ul Ferdous Department of Pharmacy, North South University, Dhaka; Department of Pharmacy, Progati Medical Institute, Dhaka, Bangladesh.
  • Manson Pandey Department of Pharmaceutical Sciences, Sardar Bhagwan Singh Post-graduate Institute of Biomedical Sciences and Research, Deheradun, India.
  • Naba Raj Pokhrel Department of Microbiology, National College, Kathmandu, Nepal.
  • Rashmi Shrestha Scheer Memorial Hospital, Banepa, Nepal.
  • Tamara Towshin Alam Department of Pharmacy, North South University, Dhaka, Bangladesh.
  • Md. Mustahsan Billah | mustahsan04pharmacy@gmail.com Department of Pharmacy, Dhaka International University, Dhaka; Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh.
  • Sayed Koushik Ahamed Department of Pharmacy, Comilla University, Comilla, Bangladesh.

Abstract

The present study determined the susceptibility to ciprofloxacin of nalidixic acid resistant Salmonella (NARS) isolated from enteric fever patients at Scheer Memorial Hospital, Banepa, Nepal, from June 2012 to December 2012. The antimicrobial sensitivity to nalidixic acid and ciprofloxacin was determined using modified Kirby-Bauer disc diffusion and broth dilution method according to the guidelines of the Clinical and Laboratory Standard Institute. Salmonella was isolated from 34 out of 992 (3.43%) blood cultures collected during the study period, and 10 (29.4%) isolates were identified as Salmonella enterica serotype Typhi, while 24 (70.6%) were identified as Salmonella enterica serotype Paratyphi. Out of the total isolates, 31 (91.2%) were nalidixic acid-resistant Salmonella (NARS). Among NARS, the minimum inhibitory concentration values for ciprofloxacin ranged from 0.25 to 2 mg/L and were constantly higher than those shown by the nalidixic acid-susceptible Salmonella. Therefore, in typhoid Salmonella nalidixic acid resistance may be the indicator of decreased susceptibility to ciprofloxacin.

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Author Biography

Girija Roka, Department of Microbiology, National College, Kathmandu
Lecturer, Dept. of Pharmacy
Published
2016-12-20
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Original Articles
Keywords:
Enteric fever, Multi-drug resistant, Nalidixic acid-resistant Salmonella, Minimum inhibitory concentration, Ciprofloxacin
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How to Cite
Roka, G., Pandaya, S., Ferdous, M. R.- ul, Pandey, M., Pokhrel, N. R., Shrestha, R., Alam, T. T., Billah, M. M., & Ahamed, S. K. (2016). Susceptibility to patterns of ciprofloxacin among nalidixic acid-resistant Salmonella isolates collected in Banepa, Nepal from enteric fever patients. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 89(2). https://doi.org/10.4081/jbr.2016.5527