Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria


Submitted: September 30, 2014
Accepted: September 30, 2014
Published: January 30, 2009
Abstract Views: 1119
PDF: 895
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay and Neutral Red Uptake (NRU). Low cytotoxic effects were observed with NRU, while MTT showed a dose-dependent effect, with a "plateau" at highest protein concentrations. Therefore, crude extraxts didn't show effect on lysosomes but MTT test emphasized cell survival decrease and a "plateau" effect at high extract doses which could be related with the inhibition of the mitochondrial dehydrogenase activity.

Parodi, A., Bassi, A., Catalano, M., Giacco, E., Mariottini, G., & Pane, L. (2009). Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 82(1). https://doi.org/10.4081/jbr.2009.4734

Downloads

Download data is not yet available.

Citations