Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria

Abstract

Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay and Neutral Red Uptake (NRU). Low cytotoxic effects were observed with NRU, while MTT showed a dose-dependent effect, with a "plateau" at highest protein concentrations. Therefore, crude extraxts didn't show effect on lysosomes but MTT test emphasized cell survival decrease and a "plateau" effect at high extract doses which could be related with the inhibition of the mitochondrial dehydrogenase activity.

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Published
2009-01-30
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Abstract Book
Keywords:
Plankton, jellyfish, keratinocytes, cytotoxicity
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How to Cite
Parodi, A., Bassi, A., Catalano, M., Giacco, E., Mariottini, G., & Pane, L. (2009). Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 82(1). https://doi.org/10.4081/jbr.2009.4734

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