CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation

  • S. Laurent
  • P. Carrega
  • D. Saverino
  • P. Piccioli
  • M. Camoriamo
  • A. Morabito
  • B. Dozin
  • V. Fontana
  • R. Simone
  • L. Mortara
  • M.C. Mingari
  • G. Ferlazzo
  • M.P. Pistillo | mariapia.pistillo@istge.it

Abstract

CTLA-4 is the major nefative regulator of T cell response. We have analyzed the expression of CTLA-4 in human monocytes and monocyte-derived DCs and the effects of its engagement on cytokine production and T cell stimulatory activity by mature DCs (mDCs). We found the CTLA-4 was highly expressed on freshly isolated monocytes, then down-modulated on the immature DCs (iDCs) and upregulated on mDCs. Treatment of mDCS with an agonistic anti-CTLA-4 m Ab enhanced secretion of IL-10 but reduced secretion of IL-8 and IL-12, as well as autologous CD4* T-cell proliferation in response to simulation with PPD recall antigenloaded-DCs. Neutralization of IL-10 with an anti-IL-10 antibody partially restored the ability of anti-CTLA-4-treated mDCs to stimulate T cell proliferation in response to PPD. Our data provide the first evidence that CTLA-4 receptor is expressed by human mDCs and exerts immune modulatory effects in these cells.

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Published
2011-01-31
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Abstract Book
Keywords:
CTLA-4, DC, cytokine secretion, T cell proliferation
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How to Cite
Laurent, S., Carrega, P., Saverino, D., Piccioli, P., Camoriamo, M., Morabito, A., Dozin, B., Fontana, V., Simone, R., Mortara, L., Mingari, M., Ferlazzo, G., & Pistillo, M. (2011). CTLA-4 expressed by human dendritic cells modulates thei cytokine secretion and induction of T cell proliferation. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 84(1). https://doi.org/10.4081/jbr.2011.4651

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