New perspective in the assessment of total intracellular magnesium


Submitted: November 21, 2013
Accepted: November 21, 2013
Published: January 10, 2014
Abstract Views: 988
PDF: 1121
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Magnesium (Mg) is essential for biological processes, but its cellular homeostasis has not been thoroughly elucidated, mainly because of the inadequacy of the available techniques to map intracellular Mg distribution. Recently, particular interest has been raised by a new family of fluorescent probes, diaza-18-crown-hydroxyquinoline (DCHQ), that shows remarkably high affinity and specificity for Mg, thus permitting the detection of the total intracellular Mg. The data obtained by fluori- metric and cytofluorimetric assays performed with DCHQ5 are in good agreement with atomic absorption spectroscopy, confirming that DCHQ5 probe allows both qualitative and quantitative determination of total intracellular Mg.

Sargenti, A., Merolle, L., Andreani, G., Cappadone, C., Farruggia, G., Iotti, S., & Marraccini, C. (2014). New perspective in the assessment of total intracellular magnesium. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2140

Downloads

Download data is not yet available.

Citations