New perspective in the assessment of total intracellular magnesium

  • Azzurra Sargenti | azzurra.sargenti@unibo.it Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
  • Lucia Merolle Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
  • Giulia Andreani Department of Veterinary Medical Sciences, University of Bologna, Italy.
  • Concettina Cappadone Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
  • Giovanna Farruggia National Institute of Biostructures and Biosystems, Roma, Italy.
  • Stefano Iotti National Institute of Biostructures and Biosystems, Roma, Italy.
  • Chiara Marraccini Department of Life Sciences, University of Modena and Reggio Emilia, Italy.

Abstract

Magnesium (Mg) is essential for biological processes, but its cellular homeostasis has not been thoroughly elucidated, mainly because of the inadequacy of the available techniques to map intracellular Mg distribution. Recently, particular interest has been raised by a new family of fluorescent probes, diaza-18-crown-hydroxyquinoline (DCHQ), that shows remarkably high affinity and specificity for Mg, thus permitting the detection of the total intracellular Mg. The data obtained by fluori- metric and cytofluorimetric assays performed with DCHQ5 are in good agreement with atomic absorption spectroscopy, confirming that DCHQ5 probe allows both qualitative and quantitative determination of total intracellular Mg.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2014-01-10
Keywords:
total intracellular magnesium, biological process, DCHQ
Statistics
  • Abstract views: 768

  • PDF: 900
How to Cite
Sargenti, A., Merolle, L., Andreani, G., Cappadone, C., Farruggia, G., Iotti, S., & Marraccini, C. (2014). New perspective in the assessment of total intracellular magnesium. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2140