Quantitative determination of quinolones residues in milk by HPLC-FLD

  • Marilena Gili | marilena.gili@izsto.it Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.
  • Daniela Marchis Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.
  • Paola Stella Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.
  • Fabio Olivo Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.
  • Federica Ostorero Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.
  • Mauro Franzoni Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.
  • Maria Cesarina Abete Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta - Torino, Italia, Italy.

Abstract

Veterinary drugs have become an integral part of the livestock production and play an important role in maintenance of animal welfare. The use of veterinary medicines may be cause of the presence of drug residues in animal food products if appropriate withdrawal periods are not respected or if contaminated feeds are used. This work presents the development of an HPLC-FLD method for the quantitative de-tection of eight quinolones – norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, difloxacin, oxolinic acid, nalidixic acid, flumequine– in bovine milk. After deproteination and extraction with a metaphos-phoric acid 1% w/v / methanol / acetonitrile (60/20/20 v/v/v) solution, the sample is partially evaporated and cleaned up on a reversed phase SPE cartridge.The extract is analyzed using an high performance liquid chromatograph with fluorescence detector. Mean recovery ranged between 65% - 88%. All the an-alytes can be identified and quantified in the concentration range 15 - 60 μg/Kg for danofloxacin and 25 - 150 μg/Kg for the other quinolones.

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Published
2012-10-31
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Section
Short Communications
Keywords:
Quinolones, milk, HPLC-FLD.
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How to Cite
Gili, M., Marchis, D., Stella, P., Olivo, F., Ostorero, F., Franzoni, M., & Abete, M. C. (2012). Quantitative determination of quinolones residues in milk by HPLC-FLD. Italian Journal of Food Safety, 1(5), 35-38. https://doi.org/10.4081/ijfs.2012.825