METHODS COMPARISON FOR ENTEROTOXIC STAPHYLOCOCCUS AUREUS CHARACTERIZATION

  • L. Decastelli | lucia.zoppi@pagepress.org Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta., Italy.
  • A. Bellio NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino., Italy.
  • C. Nogarol NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino., Italy.
  • D.M. Bianchi Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Italy.
  • S. Gallina Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Italy.
  • T. Brunetto NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino., Italy.
  • C. Ghia NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino., Italy.
  • M. Gramaglia NRL Stafilococchi coagulasi positivi compreso S.aureus; 2 S.C. Controllo Alimenti e Igiene delle Produzioni, Torino., Italy.

Abstract

The aim of this study was to compare two different methods for enterotoxic Staphylococcus aureus characterization. 110 S.aureus strains was isolated from foods and tested with ELISA method able to detect toxins type A to E in culture medium and PCR protocols able to detect the presence of genes (sea to see; seg to sej; sep; ser) encoding for staphylococcal enterotoxins. 27 strains came out positive with ELISA; 68 resulted to have at least one encoding gene. sea and ser genes were detected respectively in 29,1% and 27,3% of strains.

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Published
2011-01-08
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Short Communications
Keywords:
Staphylococcus aureus, enterotoxin, PCR
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How to Cite
Decastelli, L., Bellio, A., Nogarol, C., Bianchi, D., Gallina, S., Brunetto, T., Ghia, C., & Gramaglia, M. (2011). METHODS COMPARISON FOR ENTEROTOXIC STAPHYLOCOCCUS AUREUS CHARACTERIZATION. Italian Journal of Food Safety, 1(1zero), 263-264. https://doi.org/10.4081/ijfs.2011.880