SURVEY ON V. CHOLERAE, V. VULNIFICUS AND V. PARAHAEMOLYTICUS IN BIVALVE MOLLUSCS OF THE ADRIATIC SEA AND PROPOSAL OF AN ANALYTICAL PROTOCOL

  • P. Serratore | lucia.zoppi@pagepress.org Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
  • A. Piano Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
  • S. Piraccini Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
  • M. Trentini Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
  • E. Zavatta Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
  • M. Grodzki Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
  • M.L. Valeri Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .

Abstract

Bivalve molluscs from Adriatic sea were analyzed for V. parahaemolyticus, V. cholerae e V. vulnificus presence. The isolates on TCBS Agar and m-CPC Agar were selected on the basis of a new biochemical screening, that showed a good performance, because among 2344 strains from primary culture only 237 (10%) were presumptively assigned to the species of interest. The PCR analyses was performed for the target genes toxR hlyA, ctxA, tcpI (V. cholerae), toxR, tl, tdh, trh (V. parahaemolyticus), vvhA and viuB (V. vulnificus). Among the 9 strains confirmed to belong to V. parahaemolyticus specie, 6 were sucrose positive. On 215 samples of molluscs only 5 resulted positive for V. parahaemolyticus being toxR+, tl+, although non pathogenic (tdh-, trh-), and none for V. cholerae e V. vulnificus.

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Published
2009-06-21
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Original Articles
Keywords:
Bivalve molluscs, Adriatic sea, V. parahaemolyticus, V. cholerae, V. vulnificus
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How to Cite
Serratore, P., Piano, A., Piraccini, S., Trentini, M., Zavatta, E., Grodzki, M., & Valeri, M. (2009). SURVEY ON V. CHOLERAE, V. VULNIFICUS AND V. PARAHAEMOLYTICUS IN BIVALVE MOLLUSCS OF THE ADRIATIC SEA AND PROPOSAL OF AN ANALYTICAL PROTOCOL. Italian Journal of Food Safety, 1(4), 33-38. https://doi.org/10.4081/ijfs.2009.1399