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Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results

Danilo Pitardi, Marzia Pezzolato, Guia Richelmi, Marilena Gili, Daniele Di Corcia, Niccolò Ciprianetti, Ivan Ferrante, Karin Santoro, Carlo Ricciardi, Elena Bozzetta
  • Danilo Pitardi
    Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy | d.pitardi@alice.it
  • Marzia Pezzolato
    Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy
  • Guia Richelmi
    Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy
  • Marilena Gili
    Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy
  • Daniele Di Corcia
    Centro Regionale Antidoping, Regione Gonzole 10/1, 10043 Orbassano (To), Italy
  • Niccolò Ciprianetti
    Politecnico di Torino - LATEMAR Unit, Department of Applied Science and Technology, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy, Italy
  • Ivan Ferrante
    Laboratorio di Tecnologie Elettrobiochimiche Miniaturizzate per l’Analisi e la Ricerca - Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy
  • Karin Santoro
    Laboratorio di Tecnologie Elettrobiochimiche Miniaturizzate per l’Analisi e la Ricerca - Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy
  • Carlo Ricciardi
    Laboratorio di Tecnologie Elettrobiochimiche Miniaturizzate per l’Analisi e la Ricerca - Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy
  • Elena Bozzetta
    Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy

Abstract

17β-estradiol is the most powerful substance with estrogenic effect, commonly used as illegal growth promoter in livestock production. To avoid health risks for consumers, sensitive, reliable and low-cost methods for quantification of extremely low concentrations of such carcinogenic residues in food are needed. Antibody-immobilised microcantilever resonators were proposed as innovative biosensors able to quantify an adsorbed target mass thanks to a shift in resonance frequency. Furthermore, the quantification of masses on the order of few picograms has recently shown to be successfully achievable with very high precision. In this study, we analysed the performance of our microcantilever sensors using extracted samples of bovine muscle from experimental animals, containing variable concentrations of 17β-estradiol (HPLC-MS/MS tested). Preliminary data showed that treated animals are correctly revealed, exhibiting large negative frequency shifts. More experiments, though, are needed to obtain a correct quantification of 17β-estradiol concentration.

Keywords

17β-Estradiol detection, Bovine muscle, Microcantilever, Biosensor

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Submitted: 2013-01-15 11:39:39
Published: 2013-06-10 10:22:11
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Copyright (c) 2013 Danilo Pitardi, Marzia Pezzolato, Guia Richelmi, Marilena Gili, Daniele Di Corcia, Niccolò Ciprianetti, Ivan Ferrante, Karin Santoro, Carlo Ricciardi, Elena Bozzetta

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